CHARACTERIZATION OF A BACTERIOPHAGE RELATED TO R-TYPE PYOCINS
CHARACTERIZATION OF A BACTERIOPHAGE RELATED TO R-TYPE PYOCINS
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DOI：
10.1128/jvi.32.3.951-957.1979
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发表时间：
1979-01-01
影响因子：
5.4
通讯作者：
KOBAYASHI, M
中科院分区：
文献类型：
--
作者：
KAGEYAMA, M;SHINOMIYA, T;KOBAYASHI, M
分离并鉴定了一种具有收缩尾部的噬菌体，该噬菌体与[铜绿假单胞菌]的 R 型脓毒素非常相似。该噬菌体命名为 PS17，通过 DEAE-纤维素色谱和 CsCl 密度梯度离心纯化。它是一种含有DNA的噬菌体，纯化后的颗粒在CsCl中的密度为1.468。该噬菌体的 DNA 在 CsCl 中的密度为 1.720，表明其 G + C 含量为 61.2%。头部呈多面体状，直径69 nm，尾部长150 nm。该噬菌体被针对5种R-型脓毒菌素的抗血清制剂中和，并且针对该噬菌体的抗血清在中和R-型脓毒菌素方面具有活性。将噬菌体 PS17 的特性与之前报道的另一种类似噬菌体 PS3 进行了比较。
A bacteriophage with a contractile tail which shows very similar features to R-type pyocins [of Pseudomonas aeruginosa] was isolated and characterized. This phage, named PS17, was purified by DEAE-cellulose chromatography and CsCl density gradients centrifugation. It was a DNA-containing phage and the density of the purified particles in CsCl was 1.468. DNA from this phage had a density of 1.720 in CsCl, indicating its G + C content to be 61.2%. The head was polyhedral, 69 nm in diameter, and the tail was 150 nm in length. This phage was neutralized by antiserum preparations against 5 R-type pyocins and the antiserum against this phage was active in neutralizing R-type pyocins. The properties of phage PS17 were compared with another similar phage, PS3, which was previously reported.