Comparative cytogenetics of bats (Chiroptera): The prevalence of Robertsonian translocations limits the power of chromosomal characters in resolving interfamily phylogenetic relationships
Comparative cytogenetics of bats (Chiroptera): The prevalence of Robertsonian translocations limits the power of chromosomal characters in resolving interfamily phylogenetic relationships
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蝙蝠（翼手目）的比较细胞遗传学：罗伯逊易位的流行限制了染色体特征在解决科间系统发育关系方面的能力
DOI：
10.1007/s10577-007-1206-2
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发表时间：
2008-03-01
影响因子：
2.6
通讯作者：
Yang, Fengtang
中科院分区：
文献类型：
--
作者：
Mao, Xiuguang;Nie, Wenhui;Yang, Fengtang
虽然翼手目的单系分类得到了许多独立研究的支持，但更高层次的系统学，如微型蝙蝠的单系分类，仍然受到形态和分子研究的争议。染色体重排作为一种罕见的基因组改变，作为分子和其他形态特征的替代品，在系统发育研究中越来越受到人们的欢迎。本文首次用比较染色体涂色法研究了巨角虫科和绒毛虫科的代表种。这些结果已被整合到已出版的比较图谱中，为评估八个蝙蝠家族代表之间的全基因组染色体同源性提供了机会。我们的结果进一步证实了罗伯逊易位在蝙蝠中的广泛存在，可能涉及全臂相互易位(WART)。为了寻找每个家族和超家族的有效细胞遗传学特征(S)，通过染色体绘制和/或显带比较确定的进化染色体重排经历了两个独立的分析：(1)利用简约的分支分析和(2)将这些染色体变化映射到从文献中获得的分子定义的系统发育树上。这两项分析都清楚地表明，同型事件的流行降低了染色体特征在解决蝙蝠家族间关系方面的可靠性。
Although the monophyly of Chiroptera is well supported by many independent studies, higher-level systematics, e.g. the monophyly of microbats, remains disputed by morphological and molecular studies. Chromosomal rearrangements, as one type of rare genomic changes, have become increasingly popular in phylogenetic studies as alternatives to molecular and other morphological characters. Here, the representatives of families Megadermatidae and Emballonuridae are studied by comparative chromosome painting for the first time. The results have been integrated into published comparative maps, providing an opportunity to assess genome-wide chromosomal homologies between the representatives of eight bat families. Our results further substantiate the wide occurrence of Robertsonian translocations in bats, with the possible involvement of whole-arm reciprocal translocations (WARTs). In order to search for valid cytogenetic signature(s) for each family and superfamily, evolutionary chromosomal rearrangements identified by chromosomal painting and/or banding comparison are subjected to two independent analyses: (1) a cladistic analysis using parsimony and (2) the mapping of these chromosomal changes onto the molecularly defined phylogenetic tree available from the literature. Both analyses clearly indicate the prevalence of homoplasic events that reduce the reliability of chromosomal characters for resolving interfamily relationships in bats.