RhoA/ROCK Signaling Is Involved in Pathological Retinal Neovascularization
RhoA/ROCK Signaling Is Involved in Pathological Retinal Neovascularization
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RhoA/ROCK 信号传导参与病理性视网膜新生血管形成
DOI：
10.1159/000533321
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发表时间：
2023
影响因子：
1.7
通讯作者：
Hai
中科院分区：
文献类型：
--
作者：
F. Tang;Kongqian Huang;Bi;Wen Deng;Ning Su;Fan Xu;Mingyuan Zhang;Hai
目的：研究RhoA/ROCK抑制剂法舒地尔对视网膜新生血管（NV）和体外血管生成的影响。方法：采用C57BL/6建立OIR模型。首先，首先检测并比较了OIR和健康对照之间RhoA/ROCK的表达。然后，我们评估法舒地尔对病理性视网膜NV的影响，并进行全片视网膜染色。量化NV面积百分比、新生血管丛数（NVT）和分支点（BP）。最后，采用人脐静脉内皮细胞（HUVECs）研究法舒地尔对血管生成的影响。结果：Real-time PCR和Western blotting显示，视网膜组织中ROCK的表达在OIR中有统计学上调。此外，我们发现法舒地尔显著降低了NV面积百分比、NVT数量和血压。此外，法舒地尔可抑制VEGF诱导的HUVECs的增殖和迁移。结论：RhoA/ROCK可能参与了OIR的发病机制。其抑制剂法舒地尔在体内和体外均能抑制视网膜新生血管生成。法舒地尔可能是一种潜在的治疗视网膜血管疾病的策略。
Objective: The aim of the study was to evaluate the effect of the RhoA/ROCK inhibitor Fasudil on retinal neovascularization (NV) in vivo and angiogenesis in vitro. Methods: C57BL/6 was used to establish an OIR model. First, RhoA/ROCK expression was first examined and compared between OIR and healthy controls. Then, we evaluated the effect of Fasudil on pathological retinal NV. Whole-mount retinal staining was performed. The percentage of NV area, the number of neovascular tufts (NVT), and branch points (BP) were quantified. Finally, human umbilical vein endothelial cells (HUVECs) were used to investigate the effect of Fasudil on angiogenesis. Results: Real-time PCR and Western blotting showed that ROCK expression in retinal tissue was statistically upregulated in OIR. Furthermore, we found that Fasudil attenuated the percentage of NV area, the number of NVT, and BP significantly. In addition, Fasudil could suppress the proliferation and migration of HUVECs induced by VEGF. Conclusions: RhoA/ROCK might be involved in the pathogenesis of OIR. And its inhibitor Fasudil could suppress retinal NV in vivo and angiogenesis in vitro. Fasudil may be a potential treatment strategy for retinal vascular diseases.
影响因子：
13.7
作者：
Tanna AP;Johnson M
通讯作者：
Johnson M
影响因子：
8.1
作者：
Geloneck, Megan M.;Chuang, Alice Z.;Mintz-Hittner, Helen A.
通讯作者：
Mintz-Hittner, Helen A.