Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus
Multiple actin-related proteins of Saccharomyces cerevisiae are present in the nucleus
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DOI：
10.1093/oxfordjournals.jbchem.a022799
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发表时间：
2000-10-01
影响因子：
2.7
通讯作者：
Mizuno, S
中科院分区：
文献类型：
--
作者：
Harata, M;Oma, Y;Mizuno, S
肌动蛋白相关蛋白（actin related proteins，Arps）是一种与骨架肌动蛋白具有相同结构但功能不同的蛋白质，在生物体中广泛存在。酿酒酵母Arps基因根据其序列和功能的相关性分为Arps 1-10，其中Arp 1与肌动蛋白最相似，Arp 10与肌动蛋白最不相似。虽然Arps 1-3及其在其他生物体中的直系同源物仅定位于细胞质中，但Arp 4（也称为Act 3）定位于细胞核中并参与转录调控。在这里，我们研究了更不同的Arps可能的核功能。我们发现Arps 5-9定位在细胞核中，但Arp 10不是。在肌动蛋白中鉴定的核输出信号在胞质Arps（Arps 1-3）中很保守，但在核Arps中不太保守。凝胶过滤色谱实验表明，核Arps的尺寸大于单体，因此存在于多蛋白复合物中。由于含有Arps的核蛋白复合物被发现负责组蛋白乙酰化和染色质重塑，这表明大多数不同的Arps通过染色质调节参与转录调控。
An increasing number of actin-related proteins (Arps), which share the basal structure with skeletal actin but possess distinct functions, have been found in a wide variety of organisms. Individual Arps of Saccharomyces cerevisiae were classified into Arps 1-10 based on the relatedness of their sequences and functions, where Arp1 is the most similar to actin, and Arp10 is the least similar. While Arps 1-3 and their orthologs in other organisms are localized exclusively in the cytoplasm, Arp4 (also known as Act3) is localized in the nucleus and is involved in transcriptional regulation. Here we examined the more divergent Arps for possible nuclear functions. We show that Arps 5-9 are localized in the nucleus, but Arp10 is not. The nuclear export signals identified in actin are well conserved in the cytoplasmic Arps, Arps 1-3, but less conserved in the nuclear Arps, Gel filtration chromatography experiments show that the nuclear Arps are larger than monomer in size and thus are present in multi-protein complexes. Since nuclear protein complexes containing Arps are found to be responsible for histone acetylation and chromatin remodeling, it is suggested that most of the divergent Arps are involved in the transcriptional regulation through chromatin modulation.