Nup96-dependent hybrid lethality occurs in a subset of species from the simulans clade of drosophila
Nup96-dependent hybrid lethality occurs in a subset of species from the simulans clade of drosophila
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DOI：
10.1534/genetics.107.072827
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发表时间：
2007-05-01
期刊：
影响因子：
3.3
通讯作者：
Barbash, Daniel A.
中科院分区：
文献类型：
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作者：
Barbash, Daniel A.
雌性黑腹果蝇与雄性黑腹果蝇杂交通常产生致命的F-1杂种；当使用混合救援菌株D. simulans Lhr(1)时，F-1雄性致死率被抑制。当这些携带Lhr(1)基因的F-1雄性与黑腹龙Nup96基因的一些突变等位基因杂合时，这些雄性的生存能力就会大大降低。我在这里表明，当使用其他杂交拯救突变来产生F雄性时，类似的依赖于Nup96的致命性模式也会发生，这表明Nup96不会通过抑制Lhr(1)拯救效应来降低杂种的生存能力。这种依赖于nup96的致死率的外显率与F，杂交拯救的外显率无关，认为这两种现象反映了遗传上独立的过程。拟象龙与另外两个姊妹种一起，形成了一个进化支系，在它们的共同祖先与黑腹龙分离之后形成。我在这里报告说，Nup96（-）降低了与这些姐妹种之一的黑斑蝶杂交的F，活力，但是。而不是另一个，毛里求斯。这些结果表明，Nup96依赖性致死率是在拟南麻象的共同祖先黑腹麻象形成后进化而来的，是由Nup96、黑腹麻象X染色体上的未知基因和未知常染色体基因相互作用引起的，至少其中一些基因在拟南麻象和谢hellia中有分化，而在毛里求斯麻象中没有分化。并讨论了Nup96与其他杂交致死基因的遗传特性。
The cross of Drosophila melanogaster females to D. simulans males typically produces lethal F-1 hybrid ;males. F-1 male lethality is suppressed when the D. simulans Lhr(1), hybrid rescue strain is used. Viability of these F-1 males carrying Lhr(1) is in turn substantially reduced when the hybrids are heterozygous for some mutant alleles of the D. melanogaster Nup96 gene. I show here that similar patterns of Nup96-dependent lethality occur when other hybrid rescue mutations are used to create F, males, demonstrating that Nup96 does not reduce hybrid viability by suppressing the Lhr(1) rescue effect. The penetrance of this Nup96-dependent lethality does not correlate with the penetrance of the F, hybrid rescue, arguing that these two phenomena reflect genetically independent processes. D. simulans, together with two additional sister species, forms a clade that speciated after the divergence of their common ancestor from D. melanogaster. I report here that Nup96(-) reduces F, viability in D. melanqgaster hybrids with one of these sister species, D. sechellia, but. not with the other, D. mauritiana. These results suggest that Nup96-dependent lethality evolved after the speciation of D. melanogaster from the common ancestor of the simulans clade and is caused by an interaction among Nup96, unknown gene (s) on the D. melanogaster X chromosome, and unknown autosomal gene(s), at least some of which have diverged in D. simulans and D. sechellia but not in D. mauritiana. The genetic properties of Nup96 are also discussed relative to other hybrid lethal genes.