Dissociation of anchorage independence from tumorigenicity in human cell hybrids
Dissociation of anchorage independence from tumorigenicity in human cell hybrids
复制标题
人类细胞杂交体中贴壁独立性与致瘤性的分离
DOI：
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发表时间：
1980
影响因子：
6.4
通讯作者：
J. Wilkinson
中科院分区：
文献类型：
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作者：
E. Stanbridge;J. Wilkinson
锚定的独立性和致瘤性之间的关联进行了研究，使用一系列的种内人类细胞杂交。这些细胞系代表非致瘤性HeLa/成纤维细胞杂交体和源自它们的致瘤性分离体。这些分离体丢失的原始染色体不超过5%。非致瘤性和致瘤性细胞群均在甲基纤维素中形成集落。殖民地的相对大小似乎是作为一个稳定的性状遗传的。在甲基纤维素中连续克隆非致瘤性杂交种导致了集落形成效率的提高，但没有选择致瘤性分离体。因此，在这个人类细胞系统中，锚定独立性的特性显然与致瘤性无关。一个辅助的观察是染色体的稳定性的杂交超过许多人口doubledge。因此，这种种内人类细胞模型提供了一种基因型和表型稳定的系统，用于检查转化和瘤形成的遗传控制。
The association between anchorage independence and tumorigenicity was examined using a series of intraspecific human cell hybrids. The cell lines represented non‐tumorigenic HeLa/fibroblast hybrids and tumorigenic segregants derived from them. These segregants had lost no more than 5% of the original chromosome complement. Both non‐tumorigenic and tumorigenic cell populations formed colonies in methyl cellulose. The relative size of the colonies seemed to be inherited as a stable trait. Serial cloning of nontumorigenic hybrids in methyl cellulose led to an enhanced efficiency of colony formation but no selection for tumoregenic segregants. Thus, the property of anchorage independence is clearly dissociated from tumorigenicity in this human cell system. An ancillary observation was the chromosomal stability of the hybrids over many population doublings. This intraspecific human cell model therefore provides a genotypically and phenotypically stable system for examination of the genetic control of transformation and neoplasia.