Identification of macrophage migration inhibitory factor (MTF) in rat peripheral nerves: its possible involvement in nerve regeneration
Identification of macrophage migration inhibitory factor (MTF) in rat peripheral nerves: its possible involvement in nerve regeneration
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DOI：
10.1016/s0925-4439(98)00086-6
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发表时间：
1999-01-06
影响因子：
6.2
通讯作者：
Nishihira, J
中科院分区：
文献类型：
--
作者：
Nishio, Y;Minami, A;Nishihira, J
巨噬细胞移动抑制因子（MIF）是一种多能性免疫调节细胞因子，参与T细胞活化和炎症反应，但在外周神经系统中的研究尚未见报道。我们在这里证明了第一次表达的MIF mRNA和MIF蛋白在大鼠坐骨神经的逆转录-聚合酶链反应，蛋白质印迹和免疫组织化学。免疫组化显示MIF阳性染色，主要见于雪旺细胞。此外，我们研究了MIF mRNA的表达在坐骨神经的北方印迹分析的情况下，神经横断。在近端和远端段，MIF mRNA的水平开始增加后12小时的神经切断。从伤后24小时到第7天，水平一直很高。在第14~21天，MIF mRNA急剧下降至切断前水平。在免疫组化中，在切断后第4天，在近端节段的轴突以及非神经元细胞中大量观察到MIF阳性染色。在远段，相反，迁移到神经纤维中的神经内膜成纤维细胞或雪旺细胞显示阳性染色的沃勒变性。虽然MIF在周围神经中的确切功能仍有待阐明，但目前的结果可能代表了与当前知识状态的重大偏离，揭示了变性-再生过程中的新功能。(C)1999 Elsevier Science B.V.保留所有权利。
Macrophage migration inhibitory factor (MIF) is known as a pluripotent immunoregulatory cytokine involved in T-cell activation and inflammatory responses; however, no study on this protein in the peripheral nervous systems has been carried out. We here demonstrated for the first time expression of MIF mRNA and MIF protein in rat sciatic nerves by reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry. Immunohistochemical analysis revealed positive staining of MIF, which was largely observed in Schwann cells. Furthermore, we examined MIF mRNA expression in the sciatic nerves by Northern blot analysis in the case of nerve transection. In both proximal and distal segments, the level of MIF mRNA started to increase 12 h after the nerve transection. The level remained high from 24 h up to day 7 after the injury. During the period from days 14 to 21, MIF mRNA sharply decreased to the pre-transection level. In immunohistochemistry, positive staining of MIF was largely observed in axons as well as non-neuronal cells in proximal segments at day 4 after transection. In the distal segments, contrastingly, endoneurial fibroblasts or Schwann cells migrating into neuronal fibers showed positive staining with Wallerian degeneration. Although the precise functions of MIF in the peripheral nerves remain to be elucidated, the present results could represent a major departure from the current state of knowledge, revealing a novel function in the degenerative-regenerative process. (C) 1999 Elsevier Science B.V. All rights reserved.