HEMATOPOIETIC COLONY-FORMING CELLS FROM MICE AFTER WOUND TRAUMA
HEMATOPOIETIC COLONY-FORMING CELLS FROM MICE AFTER WOUND TRAUMA
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DOI：
10.1016/0022-4804(85)90010-1
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发表时间：
1985-01-01
影响因子：
2.2
通讯作者：
SHEEHY, PA
中科院分区：
文献类型：
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作者：
LEDNEY, GD;STEWART, DA;SHEEHY, PA
在创伤后2周内，对皮肤创伤小鼠的后腿骨髓和脾脏的多能和祖细胞区室中产生的变化进行了检查。创伤后第一周，多能细胞（脾集落形成单位，CFU-s）在脾中显著增加，在腿骨髓中略有减少。粒细胞巨噬细胞集落形成细胞（GM-CFC）显着增加，在整个2周期间，在脾脏和增加在腿骨髓在创伤后第1周。在2周期间，脾脏中的巨噬细胞集落形成细胞（M-CFC）显着减少，而在此期间腿骨髓中的巨噬细胞集落形成细胞（M-CFC）略有升高。外周血中CFU-s和GM-CFC浓度显著升高，但M-CFC浓度未升高。受伤小鼠血清支持GM-CFC生长，但不支持M-CFC生长。CHCl 3处理可提取促生长因子。血清C-反应蛋白浓度显着增加5天的时间后伤口创伤。
The changes produced in the pluripotential and progenitor cell compartments of the hind leg bone marrow and spleen of skin-wounded mice were examined over a 2-wk post-trauma period. Pluripotent cells (colony-forming unit-spleen, CFU-s) were significantly increased in the spleen and slightly reduced in the leg marrow the first week after trauma. Granulocyte macrophage colony-forming cells (GM-CFC) were significantly increased in the spleen throughout the 2-wk period and were increased in the leg marrow during the 1st post-trauma week. Macrophage colony-forming cells (M-CFC) were significantly decreased in the spleen during the 2-wk period and were slightly elevated in the leg marrow during that time. The peripheral blood contained significantly increased concentrations of CFU-s and GM-CFC but not M-CFC. Serum of wounded mice supported growth of GM-CFC but not M-CFC. The growth-promoting factor was extractable by CHCl3 treatment. Serum C-reactive protein concentrations were significantly increased for a 5-day period after wound trauma.