An inhibitor of cell proliferation released by cultures of macrophages.
An inhibitor of cell proliferation released by cultures of macrophages.
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巨噬细胞培养物释放的细胞增殖抑制剂。
DOI：
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发表时间：
1974
影响因子：
11.1
通讯作者：
E. Unanue
中科院分区：
文献类型：
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作者：
J. Calderón;R. Williams;E. Unanue
小鼠腹腔渗出液细胞的培养液抑制EL-4白血病细胞、3T3细胞和丝裂原刺激的脾淋巴细胞的[3H]胸苷掺入和增殖。抑制EL-4白血病细胞恢复正常的增殖能力时，洗涤和正常培养基中孵育。抑制活性存在于一种低分子量物质中，该物质可通过与肿瘤细胞孵育而被吸收。该物质可透析，对胰蛋白酶消化和磷酸二酯酶处理具有抗性。腹腔渗出液中的单核吞噬细胞似乎是抑制剂的来源。在正常小鼠或注射蛋白胨或感染单核细胞增生李斯特菌的小鼠的渗出液中发现相同量的抑制物质;粘附于塑料的脾细胞释放抑制剂，但量较少。我们认为，这种抑制剂可能有助于发现的有害影响时，各种细胞，包括肿瘤的，在巨噬细胞的存在下培养。
Culture fluids from mouse peritoneal exudate cells inhibited [(3)H]thymidine incorporation by, and proliferation of, EL-4 leukemia cells, 3T3 cells, and mitogen-stimulated spleen lymphocytes. Inhibited EL-4 leukemia cells recovered their normal proliferative capacity when washed and incubated in normal medium. The inhibitory activity resided in a low-molecular-weight substance that could be absorbed by incubation with the tumor cells. This substance was dialyzable and resistant to tryptic digestion and phosphodiesterase treatment. The mononuclear phagocytes in the peritoneal exudate seemed to be the source of the inhibitor. The inhibitory material was found in the same amounts in exudates of normal mice or mice injected with peptone or infected with Listeria monocytogenes; spleen cells adherent to plastic released the inhibitor but in lesser amount. We suggest that this inhibitor may contribute to the deleterious effects found when various cells, including neoplastic ones, are cultured in the presence of macrophages.