Effects of Bisphenol S Exposure on Endocrine Functions and Reproduction of Zebrafish
Effects of Bisphenol S Exposure on Endocrine Functions and Reproduction of Zebrafish
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DOI：
10.1021/es400329t
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发表时间：
2013-08-06
影响因子：
11.4
通讯作者：
Choi, Kyungho
中科院分区：
文献类型：
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作者：
Ji, Kyunghee;Hong, Seongjin;Choi, Kyungho
双酚S（BPS）在环境和生物中的检出率很高，但其对内分泌系统的影响却知之甚少。在本研究中，成年斑马鱼对暴露于0.5，5，和50 μ g/L的BPS为21天，生殖，性类固醇激素，属于下丘脑-垂体性腺（HPG）轴的基因的转录的影响进行了研究。在随后暴露或不暴露于BPS的情况下，进一步检查了对F1代性能的不利影响。当BPS>= 0.5 μ g/L时，雌鱼的产卵量和性腺指数显著降低。雄性和雌性鱼的17 β-雌二醇血浆浓度均显著增加。然而，在雄鱼中，随着cyp 19 a的上调和cyp 17和17 β hsd转录物的下调，观察到睾酮浓度的显著降低沿着。父母暴露于BPS导致延迟和孵化率较低，即使他们孵化在干净的水。连续BPS暴露在F1胚胎导致孵化率更差，畸形率增加相比，那些没有BPS暴露。我们的观察结果表明，低水平BPS暴露可影响HPG轴的反馈调节回路，损害子代的发育。
While bisphenol S (BPS) has been frequently detected both in environment and biota, limited information is available on their effects of endocrine system. In the present study, adult zebrafish pairs were exposed to 0.5, 5, and 50 mu g/L of BPS for 21 d, and the effects on reproduction, sex steroid hormones, and transcription of the genes belonging to the hypothalamic-pituitarygonad (HPG) axis were investigated. The adverse effects on performances of F1 generation were further examined with or without subsequent exposure to BPS. Egg production and the gonadosomatic index in female fish were significantly decreased at >= 0.5 mu g/L BPS. Plasma concentrations of 17 beta-estraoliol were significantly increased in both male and female fish. In male fish, however, significant decreases of testosterone concentration were observed along with up-regulation of cyp19a and down-regulation of cyp17 and 17 beta hsd transcripts. Parental exposure to BPS resulted in delayed and lesser rates of hatching even when they were hatched in clean water. Continuous BPS exposure in the F1 embryos resulted in worse hatchability and increased malformation rates compared to those without BPS exposure. Our observations showed that exposure to low level BPS could affect the feedback regulatory circuits of HPG axis and impair the development of offspring.