A novel molecular marker of pituitary tumor transforming gene involves in a rat liver regeneration
A novel molecular marker of pituitary tumor transforming gene involves in a rat liver regeneration
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DOI：
10.1016/j.jss.2005.04.019
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发表时间：
2005-11-01
影响因子：
2.2
通讯作者：
Yamashita, S
中科院分区：
文献类型：
--
作者：
Akino, K;Akita, S;Yamashita, S
背景。垂体肿瘤转化基因（PTTG）与哺乳动物苏金蛋白同源，在细胞转化中发挥着关键作用，然而，其在正常组织中的生物学功能尚不完全清楚。由于肝脏是一个再生器官，因此了解PTTG在肝脏中的相关生物学功能将更有可能了解PTTG。此外，PTTG可能参与肝再生。材料和方法。体外和体内检测大鼠肝再生细胞增殖期PTTG信使RNA(mRNA)的表达和细胞免疫反应性。结果。大鼠原代肝细胞的 PTTG 表达受到 HGF 以剂量依赖性方式刺激，并且当转化生长因子-β 1 抑制肝细胞增殖时，PTTG 表达受到抑制。通过细胞角蛋白 18 免疫荧光标记 PTTG，发现肝细胞核中与 5-溴-2'-脱氧尿苷 (BrdU) 共定位的阳性 PTTG 免疫反应性，并且存在同时的姐妹染色质本身(CK18).讨论。由于在原代大鼠培养的肝细胞中观察到 PTTG mRNA 表达、细胞增殖和免疫反应性的相关性，因此 PTTG 可能是体外和体内肝再生细胞增殖的新标志物。 (c) 2005 Elsevier Inc. 保留所有权利。
Background. Pituitary tumor transforming gene (PTTG), homologous to a mammalian securin, plays a pivotal role in cell transformation, however, its biological function(s) in normal tissues is not fully understood. Because the liver is a regenerative organ, the relevant biological function of PTTG in the liver would be more feasible to understand PTTG. Also, PTTG may be involved in the liver regeneration.Materials and methods. Expressions of rat hepatic PTTG messengerRNA (mRNA) and cellular immunoreactivities during the cell proliferative period of the liver regeneration both in vitro and in vivo were tested.Results. PTTG expression of the rat primary hepatocyte was stimulated by HGF in a dose dependent manner, and was suppressed when hepatocyte proliferation was inhibited by transforming growth factor-beta 1. A positive PTTG immunoreactive co-localizing with 5-bromo-2'-deoxyuridine (BrdU) in the hepatocyte nucleus was found and there was a concurrent sister chromatin itself by the immunofluorescent labeling of PTTG with cytokeratin 18 (CK18).Discussion. Since the correlation of PTTG mRNA expression, cell proliferation and immunoreactivity were observed in primary rat cultured hepatocytes, PTTG may be a novel marker of cell proliferation both in vitro and in vivo liver regeneration. (c) 2005 Elsevier Inc. All rights reserved.