Establishment of the DU.528 human lymphohemopoietic stem cell line.
Establishment of the DU.528 human lymphohemopoietic stem cell line.
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建立DU.528人类淋巴细胞干细胞系。
DOI：
10.1084/jem.162.5.1561
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发表时间：
1985-11-01
影响因子：
15.3
通讯作者：
Hershfield, M S
中科院分区：
文献类型：
--
作者：
Kurtzberg, J;Bigner, S H;Hershfield, M S
我们已经建立了DU.528细胞系从预处理白血病细胞的患者谁经历了T淋巴细胞转化为早幼粒细胞表型转换过程中与腺苷脱氨酶抑制剂，脱氧coformycin治疗。通过有限稀释从其获得的细胞系和克隆具有先前在患者的预处理T淋巴母细胞和脱氧共形霉素处理后的早幼粒细胞中发现的相同核型。连续培养2年以上的DU. 528细胞显示出主要的未分化T淋巴母细胞样表型。这些细胞自发产生至少三种谱系的后代，T淋巴细胞、粒细胞/单核细胞和红细胞。DU.528细胞在未分化状态下最一致表达的表面标志物是3A 1抗原，其已在胚胎胸腺中的原胸腺细胞上发现。一些未分化的DU.528细胞也表达IL-2受体，但不表达其他T细胞分化抗原。DU.528细胞暴露于各种试剂诱导髓系成熟;腺苷和脱氧腺苷，在脱氧共福霉素的存在下，诱导髓系分化抗原的表达。我们的结果表明DU.528是一种淋巴造血干细胞系，并支持多能干细胞的分化可能因腺苷脱氨酶的遗传缺陷而改变的假设。DU.528细胞可能为检查调节干细胞增殖和分化的因素提供有用的模型。
We have established the DU.528 cell line from the pretreatment leukemia cells of a patient who underwent a T lymphoblastic-to-promyelocytic phenotype conversion during treatment with the adenosine deaminase inhibitor, deoxycoformycin. The cell line and clones obtained from it by limiting dilution have the same karyotype previously found in the patient's pretreatment T lymphoblasts and post-deoxycoformycin treatment promyelocytes. DU.528 cells in continuous culture for greater than 2 yr display a predominant undifferentiated T lymphoblastoid phenotype. These cells spontaneously generate progeny of at least three lineages, T lymphoid, granulocytic/monocytic, and erythroid. The surface marker most consistently expressed by DU.528 cells in the undifferentiated state is the 3A1 antigen, which has been found on prothymocytes in the embryonic thymus. Some undifferentiated DU.528 cells also expressed the IL-2 receptor, but no other T cell differentiation antigens. Exposure of DU.528 cells to a variety of agents induced myeloid maturation; adenosine and deoxyadenosine, in the presence of deoxycoformycin, induced expression of myeloid differentiation antigens. Our results suggest that DU.528 is a lymphohematopoietic stem cell line and support the hypothesis that differentiation of pluripotent stem cells may be altered by genetic deficiency of adenosine deaminase. DU.528 cells may provide a useful model for examining factors that regulate stem cell proliferation and differentiation.