SURFACE THERMODYNAMICS OF BACTERIAL ADHESION
SURFACE THERMODYNAMICS OF BACTERIAL ADHESION
复制标题
DOI：
10.1128/aem.46.1.90-97.1983
复制
发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
NEUMANN, AW
中科院分区：
文献类型：
--
作者：
ABSOLOM, DR;LAMBERTI, FV;NEUMANN, AW
金黄色葡萄球菌049株、S.表皮葡萄球菌（菌株047）、大肠杆菌（菌株055和2627）和单核细胞增生李斯特菌（Listeria monocytogenes））对各种聚合物表面的粘附。实验方案的设计由热力学考虑决定。从悬浮液中的小颗粒粘附到固体基质上的热力学模型可以得出，粘附的程度取决于所涉及的所有3个相的表面性质，即，粘附颗粒、基底和悬浮液体介质的表面张力。实质上，粘附性更广泛地粘附于亲水性基质（即，当细菌的表面张力大于悬浮介质的表面张力时，相对高表面张力的基质）比疏水性基质更适合于细菌。当悬浮液的表面张力大于细菌的表面张力时，则相反的行为模式占主导地位。将浓度为108个微生物/ml的细菌悬浮液与几种聚合物表面（特氟隆、聚乙烯、聚苯乙烯和缩醛和磺化聚苯乙烯）在20 ℃下接触30分钟。C.冲洗后，通过图像分析确定每单位表面积粘附的细菌数量。通过添加不同量的二甲基亚砜来改变悬浮介质Hanks平衡盐溶液的表面张力。每单位表面积粘附的细菌数量与热力学预测相关性良好;因此，这些数据可用于确定不同细菌物种的表面张力。以这种方式获得的细菌的表面张力与通过其他方法获得的表面张力非常一致。
The adhesion of Staphylococcus aureus (strain 049), S. epidermidis (strain 047), Escherichia coli (strains 055 and 2627) and Listeria monocytogenes) to various polymeric surfaces was studied. The design of the experimental protocol was dictated by thermodynamic considerations. From the thermodynamic model for the adhesion of small particles from a suspension onto a solid substratum, it follows that the extent of adhesion is determined by the surface properties of all 3 phases involved, i.e., the surface tensions of the adhering particles, the substrate and the suspending liquid medium. In essence, adhesion is more extensive to hydrophilic substrata (i.e., substrata of relatively high surface tension) than to hydrophobic substrata when the surface tension of the bacteria is larger than that of the suspending medium. When the surface tension of the suspending liquid is larger than that of the bacteria, the opposite pattern of behavior prevails. Suspensions of bacteria at a concentration of 108 microorganisms/ml were brought into contact with several polymeric surfaces (Teflon, polyethylene, polystyrene and acetal and sulfonated polystyrene) for 30 min at 20.degree. C. After rinsing, the number of bacteria adhering per unit surface area was determined by image analysis. The surface tension of the suspending medium, Hanks balanced salt solution, was modified through the addition of various amounts of dimethyl sulfoxide. The number of bacteria adhering per unit surface area correlated well with the thermodynamic predictions; thus, these data may be used to determine the surface tension of the different bacterial species. The surface tensions of the bacteria obtained in this fashion are in excellent agreement with those obtained by other methods.