Core-shell-satellite microspheres-modified glass capillary for microsampling and ultrasensitive SERS spectroscopic detection of methotrexate in serum
Core-shell-satellite microspheres-modified glass capillary for microsampling and ultrasensitive SERS spectroscopic detection of methotrexate in serum
复制标题
核壳卫星微球修饰玻璃毛细管用于血清中甲氨蝶呤的微量取样和超灵敏SERS光谱检测
DOI：
10.1016/j.snb.2018.08.048
复制
发表时间：
2018-12-01
影响因子：
8.4
通讯作者：
Chen, Xiaoqing
中科院分区：
文献类型：
--
作者：
Chen, Miao;Tang, Jiading;Chen, Xiaoqing
建立了一种高灵敏、高重现性的新型表面增强拉曼散射（Sers）毛细管平台，用于血清中甲氨蝶呤（MTA）的现场检测。采用简单的镜面反应和自组装方法，在玻璃毛细管上制备了核-壳-卫星微球（CSSM）复合涂层。通过检测4-巯基苯甲酸（4-MBA）的Sers信号，可以方便地评价和控制所制备的毛细管的质量。CSSM修饰的玻璃毛细管由于静电相互作用而表现出对MTA的强烈富集、归因于粒子间等离子体耦合的高Sers性能以及对4-MBA强信号的刺激，使得能够检测低浓度的MTA分子。所制造的毛细管允许通过简易的“抽吸-测量”检测模式快速检测MTA。基于MTA和4-MBA的固有Sers光谱，在0.1 nM-110 nM范围内，MTA和4-MBA的强度比与MTA浓度的相关系数为0.990。考虑到静电力和毛细管作用，这种开发的便携式SERS使能设备在床旁（POC）诊断的实际应用中具有很大的潜力。
An ultrasensitive and highly reproducible novel surface-enhanced Raman scattering (SERS)-enabled capillary platform was developed for on-site detection of methotrexate (MTA) in serum. By employing a facile mirror reaction and a self-assembly procedure, glass capillaries were compactly coated with core-shell-satellite microspheres (CSSM) via a batch-fabrication process without using any pre-surface modifications or specialized equipment. The quality of the prepared capillaries was facilely evaluated and controlled by detecting SERS signals of 4-mercaptobenzoic acid (4-MBA) that were specifically anchored in the middle gap of CSSM. The CSSM-modified glass capillaries exhibit strong enrichment of MTA due to electrostatic interaction, high SERS performance ascribed to the interparticle plasmonic coupling, and the stimulation of strong signal of 4-MBA, enabling the detection of MTA molecules at low concentrations. The fabricated capillaries allow rapid detection of MTA via a facile "aspiration-measure" detection mode. Based on the inherent SERS spectra of MTA and 4-MBA, the intensity ratio of MTA and 4-MBA versus the concentration of MTA shows a correlation coefficient of 0.990 in the range of 0.1 nM-110 nM. Given electrostatic force and capillary action, this developed portable SERS-enabled device holds great potential for practical applications in point-of-care (POC) diagnosis.