MKLP1 requires specific domains for its dendritic targeting
MKLP1 requires specific domains for its dendritic targeting
复制标题
MKLP1 需要特定的域来实现其树突靶向
DOI：
10.1242/jcs.02750
复制
发表时间：
2006-02-01
影响因子：
4
通讯作者：
Chen, YZ
中科院分区：
文献类型：
--
作者：
Xu, XH;He, C;Chen, YZ
有丝分裂驱动蛋白样蛋白1（MKLP 1）特异性定位于发育神经元的树突，但其靶向机制尚不清楚。在这项研究中，MKLP 1的不同结构域在树突状靶向的作用进行了研究，通过生产一系列的增强型绿色荧光蛋白（eGFP）标记的MKLP 1及其变体突变，并研究这些分子在培养的原代海马神经元的分布，使用荧光显微镜。我们发现：（a）全长MKLP 1（1-856）-eGFP分布于海马神经元的树突，而不是轴突;（B）“马达”结构域的缺失阻止了树突的分布;（c）“尾”结构域的缺失导致轴突的出现;（d）缺乏“茎”结构域的突变体仍然特异性地分布于树突。结果表明，MKLP 1的运动区和尾区对于其在树突上的定位是重要的。我们还讨论了膜锚定蛋白和驱动蛋白样蛋白的靶向之间的差异。
The mitotic kinesin-like protein 1 (MKLP1) is specifically localized to the dendrite of the developed neuron, but its targeting mechanism is still unclear. In this study, the role of distinct domains of MKLP1 in dendritic targeting was investigated by producing a series of enhanced green fluorescent protein (eGFP)-tagged MKLP1 and its variant mutations, and studying the distribution of these molecules in cultured primary hippocampal neurons using fluorescence microscopy. We have found that: (a) full-length MKLP1(1-856)-eGFP was distributed to the dendrite of hippocampal neurons, not the axon; (b) deletion of the `motor' domain prevented the dendritic distribution; (c) deletion of the `tail' domain caused axonal appearance; (d) mutants devoid of the `stalk' domain were still specifically distributed to the dendrite. The results indicate that the motor and tail regions of MKLP1 are important and significant for its localization to the dendrites. We also discuss the difference between the targeting of membrane-anchoring proteins and the kinesin-like protein.