Electrophysiological basis of mono-phasic action potential recordings
Electrophysiological basis of mono-phasic action potential recordings
复制标题
单相动作电位记录的电生理学基础
DOI：
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发表时间：
1999
影响因子：
3.2
通讯作者：
Dr. L. J. Leon
中科院分区：
文献类型：
--
作者：
E. Vigmond;Dr. L. J. Leon
单相动作电位（MAPs）已有一个多世纪的记录历史，然而，其产生的确切机制尚未完全阐明。本文的目的是研究MAP记录的物理基础。通过对心脏组织的三维模块进行建模来模拟MAP记录。通过在电极下方的小区域引入一个大的非特异性漏电导来模拟MAP电极的效应。从电活动的传播中，可以有效地确定等效的细胞外电流。然后将这些计算出的电流源输入到组织的边界元模型中，以确定表面电位。最后，利用表面电位的差异来计算与MAP记录非常相似的波形。通过改变模型参数，确定了MAP的相关机制，并提出了一种能够解释所有观察结果的理论。据推测，泄漏电流导致形成一个双层电位，其强度等于电极下方区域和电极外区域之间的跨膜电压差，从而产生一个模拟电极区域外跨膜电压的记录电位，尽管存在偏移。根据实验性MAP记录，电极引入了一个等效的漏电通道，其电导为0.1 mS/cm²，反转电位为 - 43 mV。
Monophasic action potentials (MAPs)_have been recorded for over a century, however, the exact mechanism responsible for their genesis has yet to be elucidated fully. The goal of the paper is to examine the physical basis of MAP recordings. MAP recordings are simulated by modelling a three-dimensional block of cardiac tissue. The effect of the MAP electrode is modelled by introducing a large, non-specific leakage conductance to the small region under the electrode. From the spread of the electrical activity, the equivalent extracellular current flow can be efficiently determined. These computed current sources are then input into a boundary element model of the tissue to determine the surface potentials. Finally, differences in surface potentials are used to compute waveforms that closely resemble MAP recordings. By varying model parameters, the mechanisms responsible for the MAP are determined, and a theory is put forward that can account for all observations. It is hypothesised that the leakage current causes the formation of a double-layer potential with a strength equal to the difference in transmembrane voltage between the regions under the electrode and those outside the electrode, leading to a recorded potential that mimics the transmembrane voltage outside the electrode region, although offset. Based on experimental MAP recordings, an equivalent leakage channel with a conductance of 0.1 mS cm−2 and a reversal potential of −43 mV is introduced by the electrode.
影响因子：
20.1
作者：
LUO, CH;RUDY, Y
通讯作者：
RUDY, Y
影响因子：
20.1
作者：
LUO, CH;RUDY, Y
通讯作者：
RUDY, Y