HLA DPA1-DPB1 linkage disequilibrium in the British caucasoid population.
HLA DPA1-DPB1 linkage disequilibrium in the British caucasoid population.
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英国高加索人群中 HLA DPA1-DPB1 连锁不平衡。
DOI：
10.1111/j.1399-0039.1994.tb02407.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
W. Howell
中科院分区：
文献类型：
--
作者：
D. Sage;P. R. Evans;W. Howell
HLA I1类DR、DQ和DP基因具有高度多态性，编码在免疫应答中起核心作用的异二聚体细胞表面糖蛋白。HLADR和DQ基因紧密连锁，其等位基因在群体内表现出极强的连锁不平衡。然而，涉及HLA-DPB 1等位基因的I1类连锁不平衡，尽管比迄今认为的更频繁，但通常是弱的HLA-DP抗原最初使用引发淋巴细胞试验（PLT）（4）和最近使用DNA-RFLP分析和单克隆抗体（5，6）来确定。然而，测序研究和聚合酶链反应已经允许对HLA-DPAI和DPB 1基因进行更广泛的研究，并且已经鉴定出比使用细胞技术定义的多态性程度更高的多态性（7），具有多达55个WHO定义的DPB 1等位基因（8-12）。HLA-DPAI多态性更有限;然而，现在已经报道了8个DPA 1等位基因（8），并且可以使用PCR-SSOP分型来鉴定（13）。我们研究了，通过PCR-SSOP，DPA 1和DPB 1基因的等位基因频率和它们的协会在一系列的187无关的潜在的骨髓捐赠者从英国人口。用PCR引物和3组19个序列特异性寡核苷酸对DPB 1进行分型
The HLA class I1 DR DQ and DP genes are highly polymorphic and encode heterodimeric cell surface glycoproteins that play a central role in the immune response. HLA DR and DQ genes are tightly linked and the alleles of these loci show extremely strong linkage disequilibrium within populations. However, class I1 linkage disequilibrium involving HLA-DPBl alleles, although more frequent than hitherto thought, is generally weakHLA-DP antigens were initially defined using the primed lymphocyte test (PLT)(4) and more recently using DNA-RFLP analysis and monoclonal antibodies (5, 6). However, sequencing studies and the polymerase chain reaction have allowed a more extensive study of the HLA-DPAI and DPBl genes and have identified a higher degree of polymorphism than that defined using cellular techniques (7), with as many as 55 WHO-defined DPBl alleles (8-12). HLA-DPAI polymorphism is more limited; however, 8 DPAl alleles have now been reported (8) and can be identified using PCR-SSOP typing (1 3). We have studied, by PCR-SSOP, the allele frequencies of DPAl and DPBl genes and their associations in a series of 187 unrelated potential bone marrow donors from the British population. DPB 1 type was assigned using PCR primers and 3 panel of 19 sequence-specific oligonucleotide
影响因子：
4.4
作者：
A. Begovich;G. McClure;V. Suraj;R. Helmuth;N. Fildes;T. Bugawan;H. Erlich;W. Klitz
通讯作者：
A. Begovich;G. McClure;V. Suraj;R. Helmuth;N. Fildes;T. Bugawan;H. Erlich;W. Klitz