Effect of biochar amendment on the control of soil sulfonamides, antibiotic-resistant bacteria, and gene enrichment in lettuce tissues
Effect of biochar amendment on the control of soil sulfonamides, antibiotic-resistant bacteria, and gene enrichment in lettuce tissues
复制标题
生物炭改良剂对控制土壤磺胺类药物、抗生素耐药菌和生菜组织基因富集的影响
DOI：
10.1016/j.jhazmat.2015.10.074
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发表时间：
2016
影响因子：
13.6
通讯作者：
Jiang Xin
中科院分区：
文献类型：
--
作者：
Ye Mao;Sun Mingming;Feng Yanfang;Wan Jinzhong;Xie Shanni;Tian Da;Zhao Yu;Wu Jun;Hu Feng;Li Huixin;Jiang Xin
考虑到种植在抗生素污染土壤上的蔬菜通过食物链对人类健康的潜在威胁，因此迫切需要开发新的控制技术来确保蔬菜安全。通过盆栽试验，研究了生物炭改良剂对生菜组织中土壤磺胺类(SAS)、耐药菌(ARB)和ARG含量的阻抗效应。生菜种植100d后，土壤SA消散最大，生菜生长指数显著提高，残留土壤SA主要以紧密结合态形式存在。此外，根和新老叶中的SA含量比未加生物炭改良剂的降低了1~2个数量级。此外，施用生物炭后，老叶中抗金黄色葡萄球菌内生菌的分离数以及根和老叶中硫素的丰度也显著降低。但在新叶中均未检测到金黄色葡萄球菌抗性基因。这是首次研究表明，在富含ARB和ARGs的SA污染土壤上，生物炭改良剂可以成为一种保护生菜安全的实用策略。
Considering the potential threat of vegetables growing in antibiotic-polluted soil with high abundance of antibiotic-resistant genes (ARGs) against human health through the food chain, it is thus urgent to develop novel control technology to ensure vegetable safety. In the present work, pot experiments were conducted in lettuce cultivation to assess the impedance effect of biochar amendment on soil sulfonamides (SAs), antibiotic-resistant bacteria (ARB), and ARG enrichment in lettuce tissues. After 100 days of cultivation, lettuce cultivation with biochar amendment exhibited the greatest soil SA dissipation as well as the significant improvement of lettuce growth indices, with residual soil SAs mainly existing as the tightly bound fraction. Moreover, the SA contents in roots and new/old leaves were reduced by one to two orders of magnitude compared to those without biochar amendment. In addition, isolate counts for SA-resistant bacterial endophytes in old leaves andsulgene abundances in roots and old leaves also decreased significantly after biochar application. However, neither SA resistant bacteria norsulgenes were detected in new leaves. It was the first study to demonstrate that biochar amendment can be a practical strategy to protect lettuce safety growing in SA-polluted soil with rich ARB and ARGs.