Expression of the murine glutathione S-transferase α3 (GSTA3) subunit is markedly induced during adipocyte differentiation:: activation of the GSTA3 gene promoter by the pro-adipogenic eicosanoid 15-deoxy-Δ 12,14-prostaglandin J2
Expression of the murine glutathione S-transferase α3 (GSTA3) subunit is markedly induced during adipocyte differentiation:: activation of the GSTA3 gene promoter by the pro-adipogenic eicosanoid 15-deoxy-Δ 12,14-prostaglandin J2
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DOI：
10.1016/j.bbrc.2003.11.068
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发表时间：
2003-12-26
影响因子：
3.1
通讯作者：
Hayes, JD
中科院分区：
文献类型：
--
作者：
Jowsey, IR;Smith, SA;Hayes, JD
在小鼠3 T3-L1细胞的脂肪转化过程中，检查了α类、μ类和pi类谷胱甘肽S-转移酶（GST）的表达。GSTA 4、GSTM 1和GSTP 1/2亚基在融合的3 T3-L1细胞中组成型表达，并且它们的水平在脂肪细胞分化期间基本保持不变。相比之下，GSTA 3亚基在基础条件下在融合的3 T3-L1细胞中几乎检测不到，但其表达在脂肪转化期间被显著诱导。3 T3-L1脂肪形成程序的抑制表明GSTA 3表达与脂肪细胞表型的获得特异性相关。报告基因分析表明，小鼠GSTA 3 5 '-上游区域通过抗氧化反应元件被15-脱氧-Delta（12，14）-前列腺素J转录激活（2），表明这种促脂肪形成类花生酸可能参与调节脂肪形成期间GSTA 3的表达。这些数据表明，以前未认识到的作用，GST在小鼠脂肪细胞。(C)2003年爱思唯尔公司All rights reserved.
The expression of class alpha, mu, and pi glutathione S-transferases (GSTs) has been examined during the adipose conversion of mouse 3T3-L1 cells. The GSTA4, GSTM1, and GSTP1/2 subunits are expressed constitutively in confluent 3T3-L1 cells, and their levels remain essentially unchanged during adipocyte differentiation. By contrast, the GSTA3 subunit is virtually undetectable in confluent 3T3-L1 cells under basal conditions, but its expression is markedly induced during adipose conversion. Inhibition of the 3T3-L1 adipogenic program demonstrated that GSTA3 expression is associated specifically with acquisition of the adipocytic phenotype. Reporter gene assays demonstrated that the mouse GSTA3 5'-upstream region is transcriptionally activated by 15-deoxy-Delta(12,14)-prostaglandin J(2) through an antioxidant response element, suggesting that this pro-adipogenic eicosanoid may be involved in regulating GSTA3 expression during adipogenesis. These data suggest a previously unrecognised role for GSTs in mouse adipocytes. (C) 2003 Elsevier Inc. All rights reserved.