Tumor necrosis factor-alpha response to infection with Chlamydia trachomatis in human fallopian tube organ culture
Tumor necrosis factor-alpha response to infection with Chlamydia trachomatis in human fallopian tube organ culture
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DOI：
10.1016/s0002-9378(96)70035-2
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发表时间：
1996-11-01
影响因子：
9.8
通讯作者：
Terranova, PT
中科院分区：
文献类型：
--
作者：
Ault, KA;Tawfik, OW;Terranova, PT
目的：我们的目的是确定输卵管中沙眼衣原体感染是否会产生肿瘤坏死因子-α。研究设计：在腹部子宫切除术时收获输卵管，并通过标准组织培养技术进行处理。输卵管段接种沙眼衣原体血清型 E/UW-5/CX。孵育48小时后，测定上清液中的肿瘤坏死因子-α。使用免疫组织化学技术对输卵管节段进行衣原体包涵体和肿瘤坏死因子-α 染色。 结果：感染节段的平均肿瘤坏死因子-α 水平为 92.1 +/- 21.3 pg/ml（平均值 +/- SEM），对照节段为 61.9 +/- 13.9 pg/ml（配对 t 检验 p = 0.03）。肿瘤坏死因子-α 主要定位于输卵管上皮。结论：肿瘤坏死因子-α 是人类输卵管对衣原体感染的反应而产生的。它是一种重要的促炎细胞因子，可能促进其他细胞因子的产生和免疫介导的输卵管损伤。
OBJECTIVE: Our purpose was to determine whether tumor necrosis factor-alpha is produced in response to infection with Chlamydia trachomatis in the fallopian tube.STUDY DESIGN: Fallopian tubes were harvested at the time of abdominal hysterectomy and processed by standard tissue culture techniques. Tubal segments were inoculated with Chlamydia trachomatis serotype E/UW-5/CX. At 48 hours of incubation supernatant fluid was assayed for tumor necrosis factor-alpha. Tubal segments were stained for chlamydial inclusions and tumor necrosis factor-alpha by use of immunohistochemical techniques.RESULTS: Mean tumor necrosis factor-alpha levels for infected segments were 92.1 +/- 21.3 pg/ml (mean +/- SEM) and for control segments were 61.9 +/- 13.9 pg/ml (p = 0.03 by paired t test). Tumor necrosis factor-alpha was predominantly localized in the tubal epithelium.CONCLUSIONS: Tumor necrosis factor-alpha is produced in response to chlamydial infection by the human fallopian tube. It is an important proinflammatory cytokine and may promote the production of other cytokines and immune-mediated damage of the fallopian tube.