QUANTITATIVE ANALYSIS OF BIO-MEDICAL SAMPLES OF VERY SMALL QUANTITIES BY THE STANDARD-FREE METHOD
QUANTITATIVE ANALYSIS OF BIO-MEDICAL SAMPLES OF VERY SMALL QUANTITIES BY THE STANDARD-FREE METHOD
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采用无标样方法对极少量生物医学样品进行定量分析
DOI：
10.1142/s0129083597000205
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
K. Matsuda
中科院分区：
文献类型：
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作者：
K. Sera;S. Futatsugawa;S. Hatakeyama;Y. Saitoh;K. Matsuda
将自行研制的无标样方法应用于L小于1 mg或小于1μ的生物医学样品中，常规内标法无法准确应用。结果表明，NIST-牛肝样品中钾浓度的正确值接近0.5 mg。并将该方法用于少量血清和全血样品的测定，结果令人满意。对于容易从背膜上剥离的样品，如血清和全血，也可以用带有粘合剂的碳带作为背膜，发现这种方法也适用。此外，对于使用特殊设计的X射线吸收体的情况，无标准方法被证实是有效的。
A standard-free method developed by ourselves has been applied to bio-medical samples of less than 1 mg or less than 1 μl, to which an ordinary internal standard method can not be accurately applied. As the result, correct values of potassium concentration in NIST-Bovine liver samples of nearly 0.5 mg were obtained. Furthermore, the standard-free method has been applied to small quantity of serum and whole-blood samples, and satisfactory results were obtained. For samples which easily peel off from a backing film, such as serum and whole blood, a carbon tape with an adhesive agent was also used as a backing film, and it is found that this method is also applicable. Moreover, the standard-free method was confirmed to be effective for a case where a specially designed x-ray absorber is used.