Cell-permeable organic fluorescent probes for live-cell super-resolution imaging of actin filaments
Cell-permeable organic fluorescent probes for live-cell super-resolution imaging of actin filaments
复制标题
用于肌动蛋白丝活细胞超分辨率成像的细胞渗透性有机荧光探针
DOI：
10.1002/jctb.5990
复制
发表时间：
2019-06-01
影响因子：
3.4
通讯作者：
Zhang, Yu-Hui
中科院分区：
文献类型：
--
作者：
Han, Yubing;Li, Meihua;Zhang, Yu-Hui
最近发展的超分辨率显微镜已被应用于表征纳米尺度的结构的肌动蛋白丝在活细胞中，由于其在亚衍射分辨率的活细胞成像能力。然而，在现有的活细胞标记的超分辨率成像的F-肌动蛋白的方法仍然存在一些局限性。开发了一系列细胞可穿透的荧光探针，用于引入细胞穿透肽（rR）3R 2的肌动蛋白丝的超分辨率成像。这些探针是高度特异性的，易于获得的，并且适用于不同的细胞系。有了这些探针，我们解决肌动蛋白丝在60纳米的空间分辨率在活细胞超分辨率成像。我们的研究结果广泛地扩展了F-actin活细胞超分辨成像的探针类型，并为设计可渗透细胞的荧光探针开辟了新的途径。参考文献[1] Y.汉，M。Li，M. Zhang，X.黄湖，澳-地Chen，X.郝C. Kuang，X. Liu和Y.- H. Zhang，“Cell-permeable organic fluorescent probes for live-cell super-resolution imaging of actin filaments”，J.Chem.Technol.Biotechnol.，94，2040-2046（2019）。
The recently developed super-resolution microscopy has been applied to characterize the nanometer-scale structure of actin filaments in live cells, owing to its live-cell imaging ability at sub-diffraction resolution. However, there are still some limitations in existing live-cell labeling methods for super-resolution imaging of F-actin. A series of cell-permeable fluorescent probes was developed for super-resolution imaging of actin filaments with the introduction of a cell-penetrating peptide (rR)3R2. These probes are highly specific, multicolor available, and suitable for different cell lines. With these probes, we resolve actin filaments at ~60-nm spatial resolution in live-cell super-resolution imaging. Our results here extensively expand the types of probes for live-cell super-resolution imaging of F-actin and open up a new avenue in the design of cell-permeable fluorescent probes. REFERENCE [1] Y. Han, M. Li, M. Zhang, X. Huang, L. Chen, X. Hao, C. Kuang, X. Liu, and Y.-H. Zhang, “Cell-permeable organic fluorescent probes for live-cell super-resolution imaging of actin filaments,” J. Chem. Technol. Biotechnol., 94, 2040-2046 (2019).