Nocodazole induces mitotic cell death with apoptotic-like features in Saccharomyces cerevisiae
Nocodazole induces mitotic cell death with apoptotic-like features in Saccharomyces cerevisiae
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DOI：
10.1016/j.febslet.2010.04.029
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发表时间：
2010-06-03
期刊：
影响因子：
3.5
通讯作者：
Tanaka, Kozo
中科院分区：
文献类型：
--
作者：
Endo, Kingo;Mizuguchi, Mayumi;Tanaka, Kozo
我们研究了用诺考达唑（一种微管解聚药物）处理的芽殖酵母的命运。有丝分裂停滞后，细胞在有丝分裂期间死亡，表明有丝分裂细胞死亡，而不是有丝分裂滑移，主要发生在诺考达唑处理的细胞中。诺考达唑处理的细胞表现出细胞凋亡样细胞死亡的特征，但没有细胞裂解或自噬的特征。一致地，线粒体依赖性活性氧的产生与细胞死亡有关。通过扰动后期促进复合物/环体，在有丝分裂停滞后的细胞中也观察到类似的细胞死亡。此外，在诺考达唑处理的细胞中发现了半胱天冬酶活性，该活性与元半胱天冬酶 Mca1 无关。我们的结果表明，出芽酵母可以作为研究抗有丝分裂药物癌症治疗中有丝分裂细胞死亡的模型。 (C) 2010 年欧洲生化学会联合会。由 Elsevier B.V 出版。保留所有权利。
We investigated the fate of budding yeast treated with nocodazole, a microtubule-depolymerizing drug. Cells died after mitotic arrest while staying in mitosis, suggesting that mitotic cell death, but not mitotic slippage, mainly occurs in nocodazole-treated cells. Nocodazole-treated cells showed features of apoptotic-like cell death, but not those of cell lysis or autophagy. Consistently, mitochondria-dependent production of reactive oxygen species was involved in the cell death. Similar cell death was also seen in cells after mitotic arrest by perturbation of the anaphase-promoting complex/cyclosome. In addition, caspase activity was found in nocodazole-treated cells, which was independent of the metacaspase, Mca1. Our results suggest that budding yeast can be a model to study mitotic cell death in cancer treatment with antimitotic drugs. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.