flippant-An R package for the automated analysis of fluorescence-based scramblase assays.
flippant-An R package for the automated analysis of fluorescence-based scramblase assays.
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DOI：
10.1186/s12859-017-1542-y
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发表时间：
2017-03-03
影响因子：
3
通讯作者：
Graumann J
中科院分区：
文献类型：
--
作者：
Cotton RJ;Ploier B;Goren MA;Menon AK;Graumann J
蛋白质提取物和纯化的扰乱酶的脂质扰乱活性通常使用基于荧光的测定来测量。虽然该测定已经深入了解了粗膜制备物中的scramblase活性、候选scramblase的功能验证、scramblase复合物的化学计量以及翻转酶的ATP依赖性，但在其背景下的数据分析仍然是一项涉及许多手动步骤的任务。随着扩展包“flippant”的R，一个免费的软件环境的统计计算和图形，我们介绍了一个集成的解决方案的分析和出版级的图形演示连二亚硫酸盐scramblase测定，并证明其效用在重新访问原来的手动分析从出版记录，密切复制报告的结果。“Flippant”允许对乱序酶活性测定进行快速、可再现的数据分析，并提供了一个平台，用于审查、传播和扩展其所采用的策略。本文的在线版本（doi：10.1186/s12859-017-1542-y）包含补充材料，可供授权用户使用。
The lipid scrambling activity of protein extracts and purified scramblases is typically measured using a fluorescence-based assay. While the assay has yielded insight into the scramblase activity in crude membrane preparations, functional validation of candidate scramblases, stoichiometry of scramblase complexes as well as ATP-dependence of flippases, data analysis in its context has remained a task involving many manual steps. With the extension package “flippant” to R, a free software environment for statistical computing and graphics, we introduce an integrated solution for the analysis and publication-grade graphical presentation of dithionite scramblase assays and demonstrate its utility in revisiting an originally manual analysis from the publication record, closely reproducing the reported results. “flippant” allows for quick, reproducible data analysis of scramblase activity assays and provides a platform for review, dissemination and extension of the strategies it employs. The online version of this article (doi:10.1186/s12859-017-1542-y) contains supplementary material, which is available to authorized users.