Production of β-carotene by Recombinant Escherichia coli with Engineered Whole Mevalonate Pathway in Batch and Fed-batch Cultures
Production of β-carotene by Recombinant Escherichia coli with Engineered Whole Mevalonate Pathway in Batch and Fed-batch Cultures
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DOI：
10.1007/s12257-008-0230-1
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发表时间：
2009-09-01
影响因子：
3.2
通讯作者：
Kim, Chang-Joon
中科院分区：
文献类型：
--
作者：
Kim, Jung Hun;Kim, Seon-Won;Kim, Chang-Joon
Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for P-carotene biosynthesis, was utilized for production of beta-carotene in bioreactor cultures. Optimum culture conditions were established in batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific growth rate and volumetric productivity in batch cultures at 37 degrees C were 1.7-fold and 2-fold higher, respectively, than those at 28 degrees C. Glycerol was superior to glucose as a carbon source. Maximum P-carotene production (titer of 663 mg/L and overall volumetric productivity of 24.6 mg/L x h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast extract in pH-stat fed-batch culture. (C) KSBB
