Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y
Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y
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DOI：
10.1111/j.1744-7348.2009.00359.x
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发表时间：
2010-01-01
影响因子：
2.6
通讯作者：
van der Vlugt, R. A. A.
中科院分区：
文献类型：
--
作者：
Verbeek, M.;Piron, P. G. M.;van der Vlugt, R. A. A.
马铃薯Y病毒(PVY，马铃薯Y病毒属，马铃薯Y病毒科)在世界范围内造成很大的经济损失，特别是在种薯(Solanum Tuberosum)的生产上。PVY控制系统依赖于现场测量病毒压力和媒介压力。媒介压力的计算是基于蚜虫种类的相对效率因子(REFS)。这些参考文献表达了蚜虫的传播效率与烟粉虱传播效率的关系，桃蚜是PVY最有效的媒介。本文报道了PVY菌株PVYN、PVYNTN和PVYN-Wi的相对传毒效率因子(REFS)的测定。对6个PVY分离物(1个PVYN分离物、3个PVYNTN分离物和2个PVYN-Wi分离物)进行了MP2生物型的传播效率测试，结果显示所有分离物的平均传播效率相当。将该生物型在6个PVY分离物中的传播率设置为1，并以MP2为内对照，测定了6个PVY分离物中其他3个生物型的烟粉虱和16种其他种类的蚜虫(每种3个生物型)的RefS。将计算的PVYN参考文献与上个世纪报道的PVYN参考文献进行比较，我们观察到总体上具有可比性的文献，除了在本实验中参考文献较低的豆蚜、棉蚜、金星天牛、大管管蚜和管圆管虫外，以及现在参考文献较高的黄花蚜属和胡美管蚜。比较PVYNTN株的新参考文献和PVYN的新参考文献，我们观察到它们总体上是可比的，除了金黄色葡萄球菌(0.17比0.53)和禾谷裂殖吸虫(0.05比0.00)。将PVYN-Wi和PVYN计算的参考文献进行比较，可以观察到参考文献较高的6种麦长管蚜(Acyrthosiphon pisum，A.Fabae，Aphis nasturtii，Aphis spp.，P.humuli和R.Padi)。与PVYN-Wi相比，只有金针菇对PVYN-Wi的REF较低。对3种未测定REF的麦长管蚜(Aulacoreum solani、Myzus ascalonicus和S.graminum)传播PVY的能力进行了测定。
Potato virus Y (PVY, genus Potyvirus, family Potyviridae) causes high economic losses worldwide, especially in the production of seed potatoes (Solanum tuberosum). PVY control systems rely on measuring virus pressure and vector pressure in the field. Calculation of the vector pressure is based on the relative efficiency factors (REFs) of aphid species. These REFs express the transmission efficiency of aphid species in relation to the transmission efficiency of Myzus persicae, the most efficient vector of PVY. In this paper, we report on the determination of aphids' relative transmission efficiency factors (REFs) for isolates of the PVY strains PVYN, PVYNTN and PVYN-Wi. Biotype Mp2 of M. persicae was tested for its transmission efficiency for six PVY isolates (one PVYN, three PVYNTN and two PVYN-Wi isolates) and showed comparable average transmission efficiencies for all isolates. The transmission rate of this biotype for the six PVY isolates was set to 1 and Mp2 was used as an internal control in transmission experiments to determine the REFs of three other biotypes of M. persicae and 16 other aphid species (three biotypes per species when available) for the six PVY isolates. Comparing the calculated REFs for PVYN with the REFs reported in the previous century for PVYN, we observe overall comparable REFs, except for Aphis fabae, Aphis spp., Hyperomyzus lactucae, Macrosiphum euphorbiae and Rhopalosiphum padi, which have a lower REF in our experiments, and Aphis frangulae and Phorodon humuli, which have now a higher REF. Comparing the new REFs found for the PVYNTN strains with the new REFs for PVYN, we observe that they are overall comparable, except for A. frangulae (0.17 compared with 0.53) and Schizaphis graminum (0.05 compared with 0.00). Comparing the REFs calculated for PVYN-Wi with those calculated for PVYN, we can observe six aphid species with higher REFs (Acyrthosiphon pisum, A. fabae, Aphis nasturtii, Aphis spp., P. humuli and R. padi). Only the species A. frangulae shows a lower REF for PVYN-Wi compared with the transmission efficiency of PVYN. Three aphid species (Aulacorthum solani, Myzus ascalonicus and S. graminum) for which no REF was determined earlier were found to be capable to transmit PVY and their REFs were determined.