Recombinant bactericidal/permeability-increasing protein (rBPI21) in combination with sulfadiazine is active against Toxoplasma gondii.
Recombinant bactericidal/permeability-increasing protein (rBPI21) in combination with sulfadiazine is active against Toxoplasma gondii.
复制标题
重组杀菌/通透性增强蛋白 (rBPI21) 与磺胺嘧啶结合可有效对抗弓形虫。
DOI：
10.1128/aac.43.4.758
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发表时间：
1999
影响因子：
4.9
通讯作者：
Araujo,FG
中科院分区：
文献类型：
--
作者：
Khan,AA;LambertJr,LH;Remington,JS;Araujo,FG
本实验观察了重组杀菌/通透性增加蛋白（rBPI 21）单独或与磺胺嘧啶联合应用对弓形虫（Toxoplasma gondii）细胞内复制的影响。rBPI 21显著抑制T.人包皮成纤维细胞（HFF）的刚地素。作用72 h后，rBPI 21 forT. gondii的浓度为2.6 μg/ml，而在所测试的浓度下仅观察到对HFF细胞的轻微细胞毒性。随后的数学分析表明，rBPI 21与磺胺嘧啶的组合产生轻微至中度的协同效应。离体刚地菌。用C56囊肿经口或腹膜内（i. p.）用RH速殖子处理的动物死亡率为100%，而每天每公斤体重5 - 20 mg rBPI 21处理的动物死亡或显著存活时间延长（P= 0.002）。rBPI 21联合磺胺嘧啶治疗导致腹腔内感染速殖子的小鼠显著存活（P= 0.0001），但口服感染T的小鼠则无显著存活。生殖囊这些结果表明，rBPI 21在体外和体内均具有抗T活性。Gondii和当其与磺胺嘧啶组合使用时，其活性显著增强。据我们所知，这是rBPI 21抗原生动物寄生虫活性的首次报道。
The activity of recombinant bactericidal/permeability-increasing protein (rBPI21), alone or in combination with sulfadiazine, on the intracellular replication ofToxoplasma gondiiwas assessed in vitro and in mice with acute toxoplasmosis. rBPI21markedly inhibited the intracellular growth ofT. gondiiin human foreskin fibroblasts (HFFs). Following 72 h of exposure, the 50% inhibitory concentration of rBPI21forT. gondiiwas 2.6 μg/ml, whereas only slight cytotoxicity for HFF cells was observed at the concentrations tested. Subsequent mathematical analyses revealed that the combination of rBPI21with sulfadiazine yielded slight to moderate synergistic effects againstT. gondiiin vitro. Infection of mice orally with C56 cysts or intraperitoneally (i.p.) with RH tachyzoites resulted in 100% mortality, whereas prolongation of the time to death or significant survival (P= 0.002) was noted for those animals treated with 5 to 20 mg of rBPI21per kg of body weight per day. Treatment with rBPI21in combination with sulfadiazine resulted in significant (P= 0.0001) survival of mice infected i.p. with tachyzoites but not of mice infected orally withT. gondiicysts. These results indicate that rBPI21is active in vitro and in vivo againstT. gondiiand that its activity is significantly enhanced when it is used in combination with sulfadiazine. To our knowledge, this is the first report of the activity of rBPI21against a protozoan parasite.