Fabrication of antibacterial Fe3+-carboxymethylcellulose-polyacrylamide-Ag nanoparticles hydrogel coating for urinary catheters
Fabrication of antibacterial Fe3+-carboxymethylcellulose-polyacrylamide-Ag nanoparticles hydrogel coating for urinary catheters
复制标题
DOI：
10.1016/j.colsurfa.2023.131680
复制
发表时间：
2023-05
期刊：
影响因子：
--
通讯作者：
Jian-xiang Li;Han Yang;R. Gu;Yuhang Dong;Yong Cai;Qi Zhao;Yao-Cyong Chen;Mi Huang
中科院分区：
文献类型：
--
作者：
Jian-xiang Li;Han Yang;R. Gu;Yuhang Dong;Yong Cai;Qi Zhao;Yao-Cyong Chen;Mi Huang
导尿管相关性尿路感染（CAUTIs）是最常见的医院获得性感染之一。本研究采用简单浸渍法在乳胶导尿管表面制备了具有抗菌性能的Fe3+-羧甲基纤维素(CMC)-聚丙烯酰胺(PAAm) - Ag纳米颗粒（AgNPs）互穿水凝胶涂层。采用SEM、EDS、ATR-FTIR、XPS、AFM和接触角仪分别对涂层导尿管的形貌、化学成分、表面粗糙度和接触角进行了表征。通过抑菌带试验对包覆导尿管的抗菌性能进行了表征。此外，还通过体外MTT试验和溶血试验对包覆导尿管的毒性进行了评估。实验结果表明，Fe3+-CMC-PAAm-AgNPs水凝胶包被导尿管具有较强的抗菌作用。释放30天后的AgNPs浓度仍高于总Ag的有效抑菌浓度（0.1 ppb），低于人体细胞的毒性浓度（10 ppm）。包覆导尿管抗ste抑制带试验。大肠杆菌（ATCC25922）结果表明，CMC-PAAm-AgNPs水凝胶包被和Fe3+-CMC-PAAm-AgNPs水凝胶包被在培养基中有明显的抑制区。Fe3+的加入与AgNPs具有协同抑菌作用。此外，涂层还具有良好的生物相容性和血液相容性。并对其协同抗菌机理进行了探讨。新型的Fe3+-CMC-PAAm-AgNPs水凝胶导尿管涂层在减少CAUTIs方面具有很大的潜力。
Catheter-associated urinary tract infections (CAUTIs) are one of the most common hospital-acquired infections. In this study, a new Fe3+-carboxymethylcellulose (CMC)-polyacrylamide (PAAm) - Ag nanoparticles (AgNPs) interpenetrating hydrogel coating with antibacterial properties on the surfaces of latex urinary catheters was prepared with a simple dipping method. The morphology, chemical composition, surface roughness, and contact angles of the coated urinary catheters were characterized by using SEM, EDS, ATR-FTIR, XPS, AFM, and contact angle instruments, respectively. The antibacterial performance of the coated urinary catheters was characterized by the inhibition zone tests. Furthermore, the toxicity profile of the coated urinary catheters was also evaluated in vitro via MTT assay and hemolysis test. The experimental results showed that the Fe3+-CMC-PAAm-AgNPs hydrogel-coated urinary catheters had strong antibacterial effects. The concentration of AgNPs after the release of 30 days was still higher than the effective antibacterial concentration of the total Ag (0.1 ppb) and was lower than the toxic concentration of human cells (10 ppm). The inhibition zone tests of the coated urinary catheter againstE. coli(ATCC25922) showed that both the CMC-PAAm-AgNPs hydrogel coatings and the Fe3+-CMC-PAAm-AgNPs hydrogel coatings had clear inhibition zones in the culture medium. The addition of Fe3+exhibited synergistic antibacterial effects with AgNPs. Moreover, the coatings also showed admirable biocompatibility and hemocompatibility. The synergistic antibacterial mechanism was also proposed. The novel Fe3+-CMC-PAAm-AgNPs hydrogel coatings for urinary catheters would have great potential in reducing CAUTIs.