PURIFICATION OF RICKETTSIA-TSUTSUGAMUSHI BY PERCOLL DENSITY GRADIENT CENTRIFUGATION
PURIFICATION OF RICKETTSIA-TSUTSUGAMUSHI BY PERCOLL DENSITY GRADIENT CENTRIFUGATION
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DOI：
10.1111/j.1348-0421.1982.tb00181.x
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发表时间：
1982-01-01
影响因子：
2.6
通讯作者：
TSURUHARA, T
中科院分区：
文献类型：
--
作者：
TAMURA, A;URAKAMI, H;TSURUHARA, T
采用Percoll密度梯度离心法对恙虫病立克次体进行了纯化。纯化的微生物表现出良好的感染性和细胞内形态的保留。分别收获外出阶段的芽殖立克次体和增殖过程中的胞内立克次体，并利用该技术进行纯化。在电子显微镜观察中，获得的细胞内立克次体被细胞壁和细胞膜的双层膜包围，出芽立克次体被额外的最外层膜包裹，该膜可能来自出芽过程中获得的宿主细胞膜。
Purification ofRickettsia tsutsugamushihas been achieved by Percoll density gradient centrifugation. The microorganisms purified showed good retention of infectivity and intracellular morphology. Budding rickettsiae in the egressing stage and intracellular rickettsiae in the multiplying process were harvested separately and purified by this technique. In electron microscopic observations, the intracellular rickettsiae obtained were surrounded with double membrane‐layers of cell wall and cell membrane, and the budding rickettsiae were enveloped with an additional outermost membrane which may have originated from host cell membrane obtained in the budding process.