Iron speciation in the cytosol: an overview
Iron speciation in the cytosol: an overview
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DOI：
10.1039/c2dt32149a
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发表时间：
2013-01-01
影响因子：
4
通讯作者：
Kong, Xiaole
中科院分区：
文献类型：
--
作者：
Hider, Robert C.;Kong, Xiaole
虽然已经提出了一系列的候选配体和分子伴侣，但胞质铁池的性质在很大程度上仍不确定。本文综述了细胞溶质非血红素和非铁硫簇蛋白的铁结合位点以及配体对铁氧化还原活性的影响。这种分析导致的概念，铁（II）谷胱甘肽作为不稳定的胞质铁池的功能，并提供了一种手段，用于选择铁锰在随后纳入到广泛的铁依赖性酶和电子转移蛋白。谷胱甘肽和谷胱甘肽结合的谷氧还蛋白在铁硫簇合成中起关键作用，Fe（II）GS（由谷胱甘肽的巯基功能协调的铁（II））是这种生物合成途径的合适的铁供体。值得注意的是，谷胱甘肽和谷氧还蛋白都是普遍分布的，因此谷胱甘肽对细胞内铁转运的控制影响可能是大多数生物体的共同特征。
The nature of the cytosolic iron pool remains largely uncharacterized, although a range of candidate ligands and chaperones have been proposed. Herein an overview is presented of cytosolic non heme and non iron-sulphur cluster protein iron binding sites and the influence of ligands on the redox activity of iron. This analysis leads to the concept of iron(II) glutathione functioning as the labile cytosolic iron pool and offers a means for the selection of iron over manganese in subsequent incorporation into a wide range of iron-dependent enzymes and electron transfer proteins. Glutathione and glutathione-binding glutaredoxins play a critical role in iron sulfur cluster synthesis and Fe(II)GS (iron(II) coordinated by the thiol function of glutathione) is a suitable iron donor for this biosynthetic route. Significantly, both glutathione and glutaredoxins are universally distributed and thus a controlling influence of glutathione on intracellular iron trafficking is likely to be a common feature of the majority of living organisms.