Expression of genes for estrogen receptors α and β in human articular chondrocytes
Expression of genes for estrogen receptors α and β in human articular chondrocytes
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DOI：
10.1053/joca.1999.0260
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发表时间：
1999-11-01
影响因子：
7
通讯作者：
Hukuda, S
中科院分区：
文献类型：
--
作者：
Ushiyama, T;Ueyama, H;Hukuda, S
目的：探讨雌激素受体(ER) α和ER β在人关节软骨细胞中的基因表达。方法：15例患者术中取关节软骨标本16份。其中3个样本来自男性，13个来自女性；髋关节3例，膝关节13例；4例正常，12例软骨骨关节炎。从关节软骨细胞中提取总RNA，用逆转录聚合酶链反应（RT-PCR）方法检测ER α和ER β基因的表达。结果：所有标本均检测到ER α基因表达，15例标本均检测到ER β基因表达。内质网α和内质网β的数量有显著的相关性。这两种基因在男性中的表达水平明显高于女性。两种ER基因在髋关节和膝关节部位、正常组织和骨关节炎组织之间的表达水平均无显著差异。结论：据我们所知，本研究首次证实了内质网α和内质网β在人关节软骨细胞中的基因表达。由于两种受体之间存在一定的功能差异，雌激素对软骨代谢的影响应通过两种不同的受体机制来阐明。(C) 1999国际骨关节炎研究学会。
Objective: To investigate the gene expression of estrogen receptor (ER) alpha and ER beta in human articular chondrocytes.Methods: 16 articular cartilage specimens were obtained from 15 patients during surgery. Three of the specimens were from men and 13 from women; three from hip joints and 13 from knee joints; four were normal and 12 showed osteoarthritic cartilage. Total RNA was extracted from the articular chondrocytes and the expression of both ER alpha and ER beta genes was investigated by the reverse transcription-polymerase chain reaction (RT-PCR) method.Results: Gene expressions of ER alpha were detected in all specimens and those of ER beta were found in 15 specimens by the RT-PCR method. There was a significant correlation between the amounts of ER alpha and ER beta. Expression levels of both genes were significantly higher in men than in women. There were no significant differences in the expression levels of both ER genes between the hip and knee joint sites, nor between normal and osteoarthritic tissues.Conclusion: This study is to our knowledge the first to demonstrate the gene expression of both ER alpha and ER beta in human articular chondrocytes. Since there are some functional differences between the two receptors, the effects of estrogen on cartilage metabolism should be elucidated by two different receptor mechanisms. (C) 1999 OsteoArthritis Research Society International.