IDENTIFICATION OF A NEURAL ALPHA-CATENIN AS A KEY REGULATOR OF CADHERIN FUNCTION AND MULTICELLULAR ORGANIZATION
IDENTIFICATION OF A NEURAL ALPHA-CATENIN AS A KEY REGULATOR OF CADHERIN FUNCTION AND MULTICELLULAR ORGANIZATION
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DOI：
10.1016/0092-8674(92)90103-j
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发表时间：
1992-07-24
期刊：
影响因子：
64.5
通讯作者：
TAKEICHI, M
中科院分区：
文献类型：
--
作者：
HIRANO, S;KIMOTO, N;TAKEICHI, M
钙粘蛋白细胞粘附分子的功能被认为是由一组细胞质蛋白调控的，包括α -连环蛋白。我们确定了α -catenin的一个亚型，称为α -catenin，它与n -钙粘蛋白相关，主要在神经系统中表达。cDNA转染实验表明，α - n -catenin也能与E-cadherin结合。为了研究α - n -catenin的作用，我们用α - n -catenin cDNA转染了表达E-cadherin和β -catenin但不表达α -和α - n -catenin的肺癌PC9细胞。亲本PC9作为分离细胞生长时，转染细胞系形成聚集体，细胞彼此紧密粘附，呈上皮排列，偶尔产生囊性球。这些结果表明- n -连环蛋白不仅对钙粘蛋白的功能至关重要，而且对多细胞结构的组织也至关重要。
The function of cadherin cell adhesion molecules is thought to be regulated by a group of cytoplasmic proteins, including alpha-catenin. We identified a subtype of alpha-catenin, termed alpha-catenin, which is associated with N-cadherin and expressed mainly in the nervous system. cDNA transfection experiments showed that alpha-N-catenin can also bind with E-cadherin. To investigate the role of alpha-N-catenin, we transfected lung carcinoma PC9 cells, which express E-cadherin and beta-catenin but neither alpha- nor alpha-N-catenin, with alpha-N-catenin cDNA. While parental PC9 grew as isolated cells, the transfectant lines formed aggregates in which cells were tightly adhered to each other, showing epithelial arrangements, and they occasionally gave rise to cystic spheres. These results suggest that alpha-N-catenin is crucial not only for cadherin function but also for organization of multicellular structures.