Genome-resolved metagenomics of an autotrophic thiocyanate-remediating microbial bioreactor consortium.
Genome-resolved metagenomics of an autotrophic thiocyanate-remediating microbial bioreactor consortium.
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DOI：
10.1016/j.watres.2019.02.058
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发表时间：
2019-07
期刊：
影响因子：
12.8
通讯作者：
M. Watts;L. P. Spurr;K. Lê Cao;R. Wick;J. Banfield;J. Moreau
中科院分区：
文献类型：
--
作者：
M. Watts;L. P. Spurr;K. Lê Cao;R. Wick;J. Banfield;J. Moreau
黄金开采和煤炭炼焦产生的工业硫氰酸盐(SCN-−)废气污染了世界各地的环境。现代的SCN−生物修复涉及到使用复杂的工程异养微生物群，而很少有人关注简单的环境自养微生物群对SCN−的生物降解能力。在这里，我们介绍了生物反应器实验的结果，接种了SCN−的尾矿，自养培养，并受到一系列环境相关条件的影响。基因组分辨元基因组学显示，编码SCN−水解酶的硫氧化自养细菌介导了SCN−的降解。这些微生物支持代谢依赖的非SCN降解硫氧化自养生物和非硫氧化异养生物，“生态位”微生物群在空间上(浮游与固着)和时间上(跨越不断变化的环境参数)发展。随着温度的升高，生物反应器微生物组结构发生了显著变化，从硫杆菌转变为编码−水解酶的新型伽马蛋白细菌。硫杆菌CS2和COS水解酶基因是硫杆菌硫循环的关键中间体，其转化是由硫杆菌携带的CS2和COS水解酶基因介导的，揭示了这一功能水平转移的可能性。我们的工作表明，从尾矿中提取的简单自然自养微生物可以用于SCN-−生物修复，从而改善选矿水的循环利用，减少采矿的水文足迹。
Industrial thiocyanate (SCN−) waste streams from gold mining and coal coking have polluted environments worldwide. Modern SCN−bioremediation involves use of complex engineered heterotrophic microbiomes; little attention has been given to the ability of a simple environmental autotrophic microbiome to biodegrade SCN−. Here we present results from a bioreactor experiment inoculated with SCN−-loaded mine tailings, incubated autotrophically, and subjected to a range of environmentally relevant conditions. Genome-resolved metagenomics revealed that SCN−hydrolase-encoding, sulphur-oxidizing autotrophic bacteria mediated SCN−degradation. These microbes supported metabolically-dependent non-SCN--degrading sulphur-oxidizing autotrophs and non-sulphur oxidizing heterotrophs, and “niche” microbiomes developed spatially (planktonic versus sessile) and temporally (across changing environmental parameters). Bioreactor microbiome structures changed significantly with increasing temperature, shifting fromThiobacillito a novel SCN−hydrolase-encoding gammaproteobacteria. Transformation of carbonyl sulphide (COS), a key intermediate in global biogeochemical sulphur cycling, was mediated by plasmid-hosted CS2and COS hydrolase genes associated withThiobacillus, revealing a potential for horizontal transfer of this function. Our work shows that simple native autotrophic microbiomes from mine tailings can be employed for SCN−bioremediation, thus improving the recycling of ore processing waters and reducing the hydrological footprint of mining.