Tritiated thymidine autoradiographic study of cell migration and renewal in the pyloric mucosa of golden hamsters
Tritiated thymidine autoradiographic study of cell migration and renewal in the pyloric mucosa of golden hamsters
复制标题
金仓鼠幽门粘膜细胞迁移和更新的氚胸苷放射自显影研究
DOI：
10.1007/bf00220822
复制
发表时间：
1976
影响因子：
3.6
通讯作者：
S. Fujita
中科院分区：
文献类型：
--
作者：
T. Hattori;S. Fujita
SummaryThe kinetics of cell proliferation, migration and renewal in the pyloric mucosa of golden hamsters were studied by flash, cumulative and pulse labelling autoradiography following 3H-thymidine injections.By flash labelling autoradiography, it was shown that the labelled epithelial cells are exclusively confined to a zone several cells wide in the region of the isthmus between the gastric pits and the pyloric glands. In the cumulative and pulse labelling experiments, this cell proliferation in the isthmus region was shown to be for replacement of both the surface epithelial and the glandular cells. The surface epithelial cells of the pyloric mucosa arising in the upper portion of the isthmus come to line the pits and the surface, and are sloughed off into the gastric lumen within a week. The mucin-containing glandular cells, which arise more deeply in the isthmus region, migrate downwards and are apparently lost at the deepest level of the glands. The life span of the mucin-containing glandular cells was estimated at about 14 days. This cell type appears to undergo renewal of the “first produced, first lost” pipe line variety. However, a small number of glandular cells was found to survive for more than 20 days (up to 30 days), suggesting the existence of a sub-population of cells with different kinetics in the pyloric glands.
