Magnetic Cell-Scaffold Interface Constructed by Superparamagnetic IONP Enhanced Osteogenesis of Adipose-Derived Stem Cells
Magnetic Cell-Scaffold Interface Constructed by Superparamagnetic IONP Enhanced Osteogenesis of Adipose-Derived Stem Cells
复制标题
超顺磁性IONPs构建的磁性细胞支架界面增强脂肪干细胞的成骨作用
DOI：
10.1021/acsami.8b17427
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发表时间：
2018-12-26
影响因子：
9.5
通讯作者：
Zhang, Feimin
中科院分区：
文献类型：
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作者：
Chen, Huimin;Sun, Jianfei;Zhang, Feimin
组织工程和再生医学的关键因素之一是优化种子细胞与支架之间的相互作用，使细胞能够在自然仿生条件下生长。它们与大分子的相似性和许多独特的性质意味着功能性纳米颗粒具有很好的潜力，可以用于传统支架的修饰和改进，以获得优异的生物相容性，可调刚度，物理传感和刺激响应能力。在本研究中，我们报告的磁性聚（乳酸-羟基乙酸）/聚己内酯（PLGA/PCL）支架，使用组合的静电纺丝技术和超顺磁性氧化铁纳米粒子（IONP）的逐层组装。采用相同的方法制备了金纳米粒子组装的PLGA/PCL支架以进行比较。结果表明，纳米颗粒在支架表面的组装膜大大增强了支架的亲水性，提高了支架的弹性模量，从而促进了干细胞的成骨。此外，磁性的IONP被证明是促进成骨分化的关键因素，这解释了磁性支架的上级成骨能力优于金纳米颗粒组装的支架。这些结果表明磁性纳米材料作为细胞和支架之间的生物活性界面的重要性，并将促进生物材料的设计，以提高组织工程和再生医学的功效。
One of the key factors in tissue engineering and regenerative medicine is to optimize the interaction between seed cells and scaffolds such that the cells can grow in naturally biomimetic conditions. Their similarity to macromolecules and many unique properties mean that functional nanoparticles have promising potential for the modification and improvement of traditional scaffolds to obtain excellent biocompatibility, tunable stiffness, physical sensing, and stimulus-response capabilities. In the present study, we report magnetic poly(lactic-co-glycolic acid)/polycaprolactone (PLGA/PCL) scaffolds that were fabricated using a combination of the electrospinning technique and layer-by-layer assembly of superparamagnetic iron oxide nanoparticles (IONPs). PLGA/PCL scaffolds assembled with gold nanoparticles were prepared using the same method for comparison. The results showed that the assembled film of nanoparticles on the surface greatly enhanced the hydrophilicity and increased the elastic modulus of the scaffold, which subsequently improved the osteogenesis of the stem cells. Furthermore, the magnetic property of the IONPs proved to be the key factor in enhancing osteogenic differentiation, which explained the superior osteogenic capacity of the magnetic scaffolds compared with that of the gold nanoparticle-assembled scaffold. These results demonstrated the importance of magnetic nanomaterials as a bioactive interface between cells and scaffolds and will promote the design of biomaterials to improve tissue engineering and regenerative medicine efficacy.