Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility
Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility
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DOI：
10.1080/15572536.2004.11833047
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发表时间：
2003-09
期刊：
影响因子：
2.8
通讯作者：
J. Guerber;Bo Liu;J. Correll;P. Johnston
中科院分区：
文献类型：
--
作者：
J. Guerber;Bo Liu;J. Correll;P. Johnston
对被鉴定为尖孢炭疽菌（Colletotrichum acutatum）的多种分离物进行了研究，其中包括一系列果实腐烂和叶部病原体，研究内容包括线粒体DNA的限制性片段长度多态性（RFLPs）以及谷氨酰胺合成酶（GS）基因一个900bp内含子和甘油醛 - 3 - 磷酸脱氢酶（GPDH）基因一个200bp内含子的RFLPs和序列变异。线粒体DNA的RFLPs、900bp的GS内含子的RFLPs以及对每个内含子的序列分析在广义的尖孢炭疽菌中确定了相同的七个不同的分子群或分支。序列分析产生了高度一致的树形拓扑结构，明确了分支内部以及分支之间的系统发育关系。这些分支可能代表广义尖孢炭疽菌中系统发育上不同的物种。还进行了交配试验，以评估与已知能够异交形成有性型尖孢小丛壳（Glomerella acutata）的测试菌株的性兼容性。在一个分支C内以及两个系统发育不同的分支C和J4之间发现了交配兼容性。C分支包含来自多种寄主和地理来源的分离物。J4分支包含从果实腐烂以及患有顶弯病的松树幼苗中分离得到的来自澳大利亚或新西兰的分离物。两个系统发育不同的分支中的分离物能够交配，这表明基因隔离发生在生殖隔离之前。没有其他分离物与交配测试菌株有性兼容，这些测试菌株也在C和J4组中。通过线粒体DNA和内含子分析确定的某些分支（D1、J3和J6）似乎代表相对寄主受限的种群。其他分支（C1、F1和J4）包含来自多种寄主的分离物。被描述为尖孢炭疽菌松变种（C. acutatum f. sp. pineum）的分离物显然是多系的。
A diverse collection of isolates identified as Colletotrichum acutatum, including a range of fruit-rot and foliar pathogens, was examined for mtDNA RFLPs and RFLPs and sequence variation of a 900-bp intron of the glutamine synthetase (GS) gene and a 200-bp intron of the glyceraldehyde-3-phosphate dehydrogenase (GPDH) gene. RFLPs of mtDNA, RFLPs of the 900-bp GS intron and sequence analysis of each intron identified the same seven distinct molecular groups, or clades, within C. acutatum sensu lato. Sequence analysis produced highly concordant tree topologies with definitive phylogenetic relationships within and between the clades. The clades might represent phylogenetically distinct species within C. acutatum sensu lato. Mating tests also were conducted to assess sexual compatibility with tester isolates known to outcross to form the teleomorph Glomerella acutata. Mating compatibility was identified within one clade, C, and between two phylogenetically distinct clades, C and J4. The C clade represented isolates from a wide range of hosts and geographic origins. J4 clade contained isolates from Australia or New Zealand recovered from fruit rot and pine seedlings with terminal crook disease. That isolates in two phylogenetically distinct clades were capable of mating suggests that genetic isolation occurred before reproductive isolation. No other isolates were sexually compatible with the mating testers, which also were in groups C and J4. Certain clades identified by mtDNA and intron analysis (D1, J3 and J6) appeared to represent relatively host-limited populations. Other clades (C1, F1 and J4) contained isolates from a wide range of hosts. Isolates described as C. acutatum f. sp. pineum were clearly polyphyletic.