Stretching DNA using the electric field in a synthetic nanopore
Stretching DNA using the electric field in a synthetic nanopore
复制标题
DOI：
10.1021/nl0510816
复制
发表时间：
2005-10-01
期刊：
影响因子：
10.8
通讯作者：
Timp, G
中科院分区：
文献类型：
--
作者：
Heng, JB;Aksimentiev, A;Timp, G
利用电场诱导单分子通过纳米直径的孔移位的方法，检测了与蛋白质结合位置(3-10 nm)大小相当的片段上DNA的机械性能。浸泡在电解液中的DNA在电场的作用下，在10 nm厚的Si3N4膜中通过半径从0.5到1.5 nm的合成小孔。为了解释拉伸和弯曲，我们用分子动力学来模拟移位。我们已经发现了一个转位的阈值，它取决于孔的大小和所施加的跨膜偏向。电压阈值与双链DNA在60pN附近发生的伸缩转变相吻合。
The mechanical properties of DNA over segments comparable to the size of a protein-binding site (3-10 nm) are examined using an electric-field-induced translocation of single molecules through a nanometer diameter pore. DNA, immersed in an electrolyte, is forced through synthetic pores ranging from 0.5 to 1.5 nm in radius in a 10 nm thick Si3N4 membrane using an electric field. To account for the stretching and bending, we use molecular dynamics to simulate the translocation. We have found a threshold for translocation that depends on both the dimensions of the pore and the applied transmembrane bias. The voltage threshold coincides with the stretching transition that occurs in double-stranded DNA near 60 pN.