Improvement of lipid production in oleaginous yeast Rhodosporidium toruloides by ultraviolet mutagenesis
Improvement of lipid production in oleaginous yeast Rhodosporidium toruloides by ultraviolet mutagenesis
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DOI：
10.1002/elsc.201800203
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发表时间：
2019-08-01
影响因子：
2.7
通讯作者：
Chen, Jiluan
中科院分区：
文献类型：
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作者：
Guo, Minrui;Cheng, Shaobo;Chen, Jiluan
酵母Rhodosporidium toruloides AS 2.1389是一种能够积累大量油脂的工业微生物，可用于生物柴油的生产。在这项研究中，我们试图提高酵母Rhodosporidium toruloides的脂质积累的紫外线诱变和选择的基础上氯化锂耐受性或乙醇-H2 O2耐受性。突变体R.圆酵母菌属。还测定了野生型和突变株中脂质积累相关基因的转录水平。通过紫外线诱变获得耐氯化锂菌株R-ZL 2和耐乙醇-H2 O2菌株R-ZY 13。这两个突变株表现出更大的脂质生产力和脂质产量相比，野生型。转录分析显示，IDP 1，GPD 1和GND在两个高脂产生突变体中以显著更高的水平表达。因此，在不同的培养条件下，R.通过紫外诱变和选择，成功地改进了圆酵母。我们还鉴定了一些与脂质积累相关的基因，以通过基因工程提高脂质生产率。
The oleaginous yeast Rhodosporidium toruloides AS 2.1389 is viewed as desirable industrial microorganisms that can accumulate a high content of lipids for biodiesel production. In this study, we attempted to improve lipid accumulation in the yeast Rhodosporidium toruloides by UV irradiation mutagenesis and selection based on lithium chloride tolerance or ethanol-H2O2 tolerance. The biomass concentration, lipid yield and glucose consumption of mutant R. toruloides were determined. The transcription levels of lipid accumulation-related genes in the wild-type and mutant strains were also determined. The lithium chloride-tolerant strain R-ZL2 and the ethanol-H2O2-resistant strain R-ZY13 were generated by UV mutagenesis. The two mutant strains showed greater lipid productivity and lipid yield compared to the wild type. Transcriptional analysis revealed that IDP1, GPD1 and GND were expressed at significantly higher levels in the two high-lipid-producing mutants. In conclusion, lipid productivity and lipid yield in R. toruloides were successfully improved via UV mutagenesis and selection. We also identified some lipid accumulation-related genes for improving lipid productivity through genetic engineering.