DNA-binding affinities of MyoD and E47 homo- and hetero-dimers by capillary electrophoresis mobility shift assay.
DNA-binding affinities of MyoD and E47 homo- and hetero-dimers by capillary electrophoresis mobility shift assay.
复制标题
通过毛细管电泳迁移率变动分析测定 MyoD 和 E47 同二聚体和异二聚体的 DNA 结合亲和力。
DOI：
10.1016/s0021-9673(99)00923-1
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Etzkorn,FA
中科院分区：
文献类型：
--
作者：
Foulds,GJ;Etzkorn,FA
A simple capillary electrophoresis mobility shift assay (CEMSA), with no gel and uncoated capillaries, for the accurate determination of protein–DNA affinities free in solution was applied to constructs of the MyoD/E47 DNA-binding proteins. The determined affinities are compared to those obtained by EMSA. MyoD-E47 covalent heterodimer binds DNA more tightly (Kd=1.8 nM) than MyoD (Kd=14.2 nM) or E47 (Kd=11.5 nM) covalent homodimers. The effect of non-specific DNA on binding affinities was more important than salt concentration in the MyoD/E47 series. Application of this method to the MyoD/E47 system demonstrates the generality of our CEMSA.
