SNP discovery based on CATS and genotyping in the finless porpoise (Neophocaena phocaenoides)
SNP discovery based on CATS and genotyping in the finless porpoise (Neophocaena phocaenoides)
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基于 CATS 和江豚基因分型的 SNP 发现（Neophocaena phocaenoides）
DOI：
10.1007/s10592-009-9882-4
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发表时间：
2009-12-01
影响因子：
2.2
通讯作者：
Yang, Guang
中科院分区：
文献类型：
--
作者：
Li, Shuzhen;Wan, Huirong;Yang, Guang
对江豚进行了单核苷酸多态性（SNPs）发现和基因分型。利用来自人、鼠等哺乳动物基因组的202个比较锚标记序列引物对江豚种群进行了筛选。在发现的51个SNPs中，25个用理想的基因分型引物和片段长度差异等位基因特异性PCR检测进一步表征。本研究首次报道了江豚SNP位点，为江豚的保护和管理提供了新的分子标记和遗传信息。
Single nucleotide polymorphisms (SNPs) discovery and genotyping were performed for the finless porpoises (Neophocaena phocaenoides). About 202 comparative anchor tagged sequence primers derived from genomes of human, mouse and some other mammals were used to screen the finless porpoise population. Of the 51 SNPs discovered, 25 were further characterized with ideal genotyping primers and using fragment length discrepant allele specific PCR assay. This is the first report of SNP loci for the finless porpoise, which is helpful to provide some novel molecular markers and new genetic information relevant to the conservation and management of this endangered species.