ORAL PASSIVE-IMMUNIZATION EFFECT OF ANTI-HUMAN ROTAVIRUS IGY AND ITS BEHAVIOR AGAINST PROTEOLYTIC-ENZYMES
ORAL PASSIVE-IMMUNIZATION EFFECT OF ANTI-HUMAN ROTAVIRUS IGY AND ITS BEHAVIOR AGAINST PROTEOLYTIC-ENZYMES
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DOI：
10.1271/bbb.57.1077
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发表时间：
1993-07-01
影响因子：
1.6
通讯作者：
EBINA, T
中科院分区：
文献类型：
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作者：
HATTA, H;TSUDA, K;EBINA, T
抗人轮状病毒(HRV)IgY中和效价在pH 2.0时可被胃酶完全灭活。但在一定条件下，胰酶和胰凝乳酶对其无明显影响。乳鼠灌胃后2小时内即可观察到IgY的免疫活性，此后活性迅速下降。因此，口服卵黄抗体对HRV诱导的乳鼠腹泻的效果进行了评估，发现预先注射卵黄抗体(在HRV感染前1h)完全阻止了HRV诱导的腹泻。免疫间隔时间越长，预防效果越差。然而，在HRV感染后24小时内口服卵黄抗体仍然有效，并降低了哺乳小鼠HRV腹泻的发生率。
The neutralization titer of anti-human rotavirus (HRV) IgY was completely inactivated by pepsin at pH 2.0. However, it was not significantly affected by trypsin or chymotrypsin under certain conditions. The immunological activity of the IgY was observed in the intestine of suckling mice for 2h after oral administration and the activity rapidly decreased thereafter. The effects of oral supply of IgY were thus estimated for HRV-induced diarrhea in suckling mice and it was found that a previous supply of the IgY (1 h before HRV infection) completely prevented the HRV-induced diarrhea. The preventive effect was decreased as the time gap between IgY administration and HRV infection was longer. However, the oral supply of the IgY within 24h after HRV infection was still effective and decreased the incidence of HRV diarrhea in suckling mice.