Oxygen-independent poly(dimethylsiloxane)-based carbon-paste glucose biosensors
Oxygen-independent poly(dimethylsiloxane)-based carbon-paste glucose biosensors
复制标题
DOI：
10.1016/s0956-5663(02)00092-1
复制
发表时间：
2002-12-01
影响因子：
12.6
通讯作者：
Dasgupta, PK
中科院分区：
文献类型：
--
作者：
Wang, J;Li, SF;Dasgupta, PK
几种硅油已被评估和比较作为氧气的内部来源，连接到它们的粘合剂用于碳糊葡萄糖生物传感器。所有四种聚二甲基硅氧烷（PDMS）油都测试了碳糊状酶电极的氧容量急剧增加，以便在严重缺氧条件下方便地进行生物传感。由此产生的氧独立性优于全氟碳粘合剂或基于介质的生物电极。从相同的响应（在有氧和无氧情况下观察到）到2 × 10(-2) M葡萄糖和4 × 10(-2) M时的轻微（12%）灵敏度损失可以看出对氧效应的抗性。PDMS碳糊酶电极在无氧溶液中长时间（15小时）运行后显示出稳定的葡萄糖响应。在线连续测试显示良好的动态特性，在生理和病理生理范围内（3-12 mM葡萄糖）没有携带效应。(C) 2002 Elsevier Science B.V.版权所有
Several silicone oils have been assessed and compared as an internal source of oxygen in connection to their use as binders for carbon-paste glucose biosensors. All four poly(dimethylsiloxane) (PDMS) oils tested a dramatic increase in the oxygen capacity of carbon-paste enzyme electrodes to allow convenient biosensing under severe oxygen-deficit conditions. The resulting oxygen independence is better than that exerted by perfluorocarbon binders or that displayed by mediator-based bioelectrodes. The resistance to oxygen effects is indicated from the identical response (observed in the presence and absence of oxygen) up to 2 x 10(-2) M glucose and the slight (12%) sensitivity loss at 4 x 10(-2) M. The influence of the viscosity of the PDMS binder upon the internal oxygen supply is examined. The PDMS carbon-paste enzyme electrode displays a stable glucose response over prolonged (15 h) operation in an oxygen-free solution. On-line continuous testing indicates favorable dynamic properties with no carry-over effects over the physiological and pathophysiological range (3-12 mM glucose). (C) 2002 Elsevier Science B.V. All rights reserved.