The Role of VAMP7/TI-VAMP in Cell Polarity and Lysosomal Exocytosis in vivo
The Role of VAMP7/TI-VAMP in Cell Polarity and Lysosomal Exocytosis in vivo
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DOI：
10.1111/j.1600-0854.2011.01247.x
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发表时间：
2011-10-01
期刊：
影响因子：
4.5
通讯作者：
Harada, Akihiro
中科院分区：
文献类型：
--
作者：
Sato, Mahito;Yoshimura, Shinichiro;Harada, Akihiro
已经提出VAMP 7或破伤风神经毒素不敏感囊泡相关膜蛋白（TI-VAMP）调节极化上皮细胞中的顶端运输、神经元中的轴突运输和溶酶体胞吐作用。为了研究VAMP 7在体内的功能，我们产生了VAMP 7敲除小鼠。在这里，我们表明，VAMP 7敲除小鼠与对照小鼠是无法区分的，并显示在肾脏和小肠中的顶端蛋白的类似定位和神经系统中的轴突蛋白的类似定位。培养的突变海马神经元的突起生长减少突变神经元。然而，突变体成纤维细胞中的溶酶体胞吐不受影响。我们的研究结果表明，VAMP 7在神经元中是必需的，以充分延伸轴突。然而，VAMP 7似乎不需要上皮细胞极性和溶酶体胞吐。
VAMP7 or tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) has been proposed to regulate apical transport in polarized epithelial cells, axonal transport in neurons and lysosomal exocytosis. To investigate the function of VAMP7 in vivo, we generated VAMP7 knockout mice. Here, we show that VAMP7 knockout mice are indistinguishable from control mice and display a similar localization of apical proteins in the kidney and small intestine and a similar localization of axonal proteins in the nervous system. Neurite outgrowth of cultured mutant hippocampal neurons was reduced in mutant neurons. However, lysosomal exocytosis was not affected in mutant fibroblasts. Our results show that VAMP7 is required in neurons to extend axons to the full extent. However, VAMP7 does not seem to be required for epithelial cell polarity and lysosomal exocytosis.