Stochastic Collective Movement of Cells and Fingering Morphology: No Maverick Cells
Stochastic Collective Movement of Cells and Fingering Morphology: No Maverick Cells
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DOI：
10.1016/j.bpj.2009.05.064
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发表时间：
2009-10-07
影响因子：
3.4
通讯作者：
Bar-Yoseph, Pinhas Zvi
中科院分区：
文献类型：
--
作者：
Ouaknin, Gaddiel Yonathan;Bar-Yoseph, Pinhas Zvi
模拟集体生物细胞运动的经典方法是通过生物细胞和与生物细胞相互作用的扩散化学物质的耦合非线性反应扩散方程。这种方法考虑了细胞的扩散、增殖、细胞死亡和趋化性。虽然经典的方法有很多优点，但它没有考虑影响多细胞运动的许多因素。在这项工作中，多尺度的方法，Glazier-Graner-Hogeweg模型，使用。该模型是实现生物细胞与扩散化学品的有限元方法相结合。Glazier-Graner-Hogeweg模型将生物细胞状态视为离散的，并允许其包括生物细胞之间的凝聚力，细胞的变形，遵循单个细胞的路径以及细胞的随机行为。当表皮组织的连续性被破坏时，生物细胞再生皮肤以愈合伤口。我们假设当细胞感觉到受伤区域时，它们会分泌一种扩散性化学物质，并且细胞会被它们释放的化学物质吸引（趋化性）。在一定的参数下，锋面遇到指进形态，两个相对前进的锋面相互吸引并关联。细胞流动呈现出有趣的模式，化学物质的漂移效应可能会影响细胞的运动。极化基板的影响也进行了讨论。
The classical approach to model collective biological cell movement is through coupled nonlinear reaction-diffusion equations for biological cells and diffusive chemicals that interact with the biological cells. This approach takes into account the diffusion of cells, proliferation, death of cells, and chemotaxis. Whereas the classical approach has many advantages, it fails to consider many factors that affect multicell movement. In this work, a multiscale approach, the Glazier-Graner-Hogeweg model, is used. This model is implemented for biological cells coupled with the finite element method for a diffusive chemical. The Glazier-Graner-Hogeweg model takes the biological cell state as discrete and allows it to include cohesive forces between biological cells, deformation of cells, following the path of a single cell, and stochastic behavior of the cells. Where the continuity of the tissue at the epidermis is violated, biological cells regenerate skin to heal the wound. We assume that the cells secrete a diffusive chemical when they feel a wounded region and that the cells are attracted by the chemical they release (chemotaxis). Under certain parameters, the front encounters a fingering morphology, and two fronts progressing against each other are attracted and correlated. Cell flow exhibits interesting patterns, and a drift effect on the chemical may influence the cells' motion. The effects of a polarized substrate are also discussed.