Quantification of NS1 dengue biomarker in serum via optomagnetic nanocluster detection.
Quantification of NS1 dengue biomarker in serum via optomagnetic nanocluster detection.
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DOI：
10.1038/srep16145
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发表时间：
2015-11-05
影响因子：
4.6
通讯作者：
Donolato M
中科院分区：
文献类型：
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作者：
Antunes P;Watterson D;Parmvi M;Burger R;Boisen A;Young P;Cooper MA;Hansen MF;Ranzoni A;Donolato M
登革热是一种热带病媒传播的疾病，没有治愈或疫苗，逐渐蔓延到温带气候地区。在资源有限的情况下，诊断工具将是非常有价值的流行病学控制和遏制暴发。在这里，我们提出了一种新的低成本的自动化生物传感平台，用于检测登革热生物标志物NS 1，并证明它对NS 1掺入人血清。磁性纳米颗粒（MNP）通过生物正交无Cu“点击”化学在防污表面分子结构上涂覆有针对NS 1的高亲和力单克隆抗体。靶抗原NS 1的存在触发MNP凝集和纳米簇的形成，其具有通过外部磁致动增强的快速动力学。纳米团簇的量和尺寸与目标浓度相关，并且可以使用光磁读出方法进行定量。由此产生的自动登革热检测仅需8分钟，需要6 μL血清样本，检测限为25 ng/mL，检测范围上限为20000 ng/mL。该技术具有很大的潜力，可应用于患者样本中的NS 1检测。由于该测定在低成本的微流体盘上实施，该平台适合于进一步扩展到多种生物标志物的多重检测。
Dengue is a tropical vector-borne disease without cure or vaccine that progressively spreads into regions with temperate climates. Diagnostic tools amenable to resource-limited settings would be highly valuable for epidemiologic control and containment during outbreaks. Here, we present a novel low-cost automated biosensing platform for detection of dengue fever biomarker NS1 and demonstrate it on NS1 spiked in human serum. Magnetic nanoparticles (MNPs) are coated with high-affinity monoclonal antibodies against NS1 via bio-orthogonal Cu-free ‘click’ chemistry on an anti-fouling surface molecular architecture. The presence of the target antigen NS1 triggers MNP agglutination and the formation of nanoclusters with rapid kinetics enhanced by external magnetic actuation. The amount and size of the nanoclusters correlate with the target concentration and can be quantified using an optomagnetic readout method. The resulting automated dengue fever assay takes just 8 minutes, requires 6 μL of serum sample and shows a limit of detection of 25 ng/mL with an upper detection range of 20000 ng/mL. The technology holds a great potential to be applied to NS1 detection in patient samples. As the assay is implemented on a low-cost microfluidic disc the platform is suited for further expansion to multiplexed detection of a wide panel of biomarkers.