Gelatin nanoparticles produced by a simple W/O emulsion as delivery system for methotrexate
Gelatin nanoparticles produced by a simple W/O emulsion as delivery system for methotrexate
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DOI：
10.1023/a:1014791327253
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发表时间：
2002-05-01
影响因子：
3.7
通讯作者：
Zhu, ZH
中科院分区：
文献类型：
--
作者：
Cascone, MG;Lazzeri, L;Zhu, ZH
采用基于单一油包水乳液的简单溶剂蒸发技术制备了含有不同初始量甲氨蝶呤（MTX）的可生物降解亲水性明胶纳米颗粒，并使用戊二醛作为交联剂进行稳定。考察了几个参数对颗粒大小、药物包封效率和药物释放的影响。通过扫描电镜观察纳米颗粒的大小和形状。体外监测MTX的释放并研究其释放机制。制备了平均直径为100-200 nm的MTX颗粒，其释放机制为扩散控制释放机制。我们观察到，MTX的初始量对样品的释放模式没有影响，但它会影响纳米颗粒中包裹的药物量，以及药物的释放速度和释放总量。(C) 2002年Kluwer学术出版社。
Biodegradable hydrophilic gelatin nanoparticles, containing different initial amounts of methotrexate (MTX), were prepared using a simple solvent evaporation technique based on a single water-in-oil emulsion and stabilized by the use of glutaraldehyde as cross-linking agent.The effects of several parameters on particle size, drug encapsulation efficiency and drug release were investigated. Size and shape of the nanoparticles were examined by scanning electron microscopy.The release of MTX was monitored in vitro and the mechanism of release was studied. Particles with a mean diameter of 100-200 nm were produced, which were able to release MTX following a diffusion-controlled mechanism of release. It was observed that the initial amount of MTX used for sample loading did not have any effect on the pattern of release, while it affected the amount of drug entrapped into the nanoparticles and also both the release rate and the total amount of drug released. (C) 2002 Kluwer Academic Publishers.