C-MYC AND C-FOS EXPRESSION IN DIFFERENTIATING MOUSE PRIMARY KERATINOCYTES
C-MYC AND C-FOS EXPRESSION IN DIFFERENTIATING MOUSE PRIMARY KERATINOCYTES
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DOI：
10.1002/j.1460-2075.1986.tb04579.x
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发表时间：
1986-11-01
期刊：
影响因子：
11.4
通讯作者：
WEINBERG, RA
中科院分区：
文献类型：
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作者：
DOTTO, GP;GILMAN, MZ;WEINBERG, RA
在小鼠原代角质形成细胞被钙或醋酸十四烷酰磷（TPA）诱导终末分化后，myc和fos基因的表达被监测。生长细胞中的myc RNA水平非常高，即使在钙诱导分化后的后期也保持升高。因此，角化细胞提供了第一个正常垂体细胞持续表达c-myc的例子，无论其增殖或分化状态如何。Fos的表达也相对不受钙的影响。与钙相比，tpa诱导的分化伴随着原癌基因表达的急剧变化：显著的c-fos诱导和c-myc表达的显著（尽管是短暂的）下降。这些作用可能对角化细胞对TPA的反应很重要：TPA处理对这种物质有抗性的角化细胞细胞系（RBK）对c-myc表达没有影响，只导致最小的c-fos诱导。在这些细胞中，添加新鲜培养基仍可触发充分的fos诱导。因此，正常角质形成细胞中的fos基因可以通过至少两种独立的机制诱导，其中只有一种在tpa抗性细胞系的衍生过程中丢失。
Expression of the myc and fos genes has been monitored in mouse primary keratinocytes after induction of terminal differentiation by calcium or tetradecanoylphorbol acetate (TPA). myc RNA levels in growing cells are very high and remain elevated even at late times after calcium-induced differentiation. Thus, keratinocytes provide the first example of normal pituitary cells with persistent c-myc expression irrespective of their proliferative or differentiated state. fos expression is also relatively unaffected by addition of calcium. In contrast to calcium, TPA-induced differentiation is accompanied by dramatic changes in proto-oncogene expression: marked c-fos induction and considerable although transient decrease in c-myc expression. These effects might be important for the keratinocyte response to TPA: TPA treatment of a keratinocyte cell line (RBK) resistant to this substance has no effect on c-myc expression and leads only to minimal c-fos induction. In these cells full fos induction can still be triggered by addition of fresh medium. Thus, the fos gene in normal keratinocytes is inducible through at least two independent mechanisms, only one of which has been lost during derivation of the TPA-resistant cell line.