Expression of ribosomal protection proteinRppAis regulated by a ribosome-dependent ribo-regulator and two mistranslation products
Expression of ribosomal protection proteinRppAis regulated by a ribosome-dependent ribo-regulator and two mistranslation products
复制标题
核糖体保护蛋白 RppA 的表达受核糖体依赖性核糖调节因子和两种误译产物的调节
DOI：
10.1111/1462-2920.15143
复制
发表时间：
2020-07-21
影响因子：
5.1
通讯作者：
Cai, Jun
中科院分区：
文献类型：
--
作者：
Cai, Xia;Zhan, Yunda;Cai, Jun
基因表达受到转录因子和RNA调控元件的严格调控，包括反式作用的小RNA、顺式调节的核糖开关和核糖体依赖的核糖体调节因子。在本研究中，我们证明了一个核糖体依赖的核糖体调节因子和两个误翻译产物共同调节了苏云金芽孢杆菌BMB171中ppA(编码核糖体保护蛋白)的表达。TherppA基因的前导RNA通过翻译位于ITS序列中的前导ORF1来控制rppA的表达。在氯霉素存在下，ORF1的+1移码产物(ORF2)和终止密码子通读产物(ORF3)出现。ORF3对rppA的表达有负向影响。相比之下，ORF2促进了ppA的表达。本研究确定的调控模式将有助于加深对细菌基因表达的理解。
Gene expression is tightly controlled by transcription factors and RNA regulatory elements, includingtrans-acting small RNAs,cis-regulatory riboswitches and ribosome-dependent ribo-regulators. In the present study, we demonstrated that a ribosome-dependent ribo-regulator and two mistranslation products co-regulaterppA(encoding a ribosomal protection protein) expression inBacillus thuringiensisBMB171. The leader RNA of therppAgene controlsrppAexpression via translation of leader ORF1 resident in its sequence. In the presence of chloramphenicol, a +1 frameshift product (ORF2) and a stop codon readthrough product (ORF3) of ORF1 emerged. ORF3 exerted a negative effect onrppAexpression. By contrast, the ORF2 promotedrppAexpression.The regulation mode identified in the present study will lead to a deeper understanding of bacterial gene expression.