Tamoxifen-inducible cardiac-specific Cre transgenic mouse using VIPR2 intron
Tamoxifen-inducible cardiac-specific Cre transgenic mouse using VIPR2 intron
复制标题
DOI：
10.1186/s42826-020-00065-x
复制
发表时间：
2020-09-15
影响因子：
2.9
通讯作者：
Lee, Daekee
中科院分区：
文献类型：
--
作者：
Chin, Hyun Jung;Lee, So-young;Lee, Daekee
通过基因删除构建的基因工程小鼠模型是分析基因功能的有用工具。为了暂时删除某个组织中的基因，通常使用组织特异性和他莫昔芬诱导的Cre转基因小鼠。在这里，我们产生了心脏特异性表达 Cre 重组酶的转基因小鼠，该酶在人血管活性肠肽受体 2 (VIPR2) 内含子和 Hsp68 启动子 (VIPR2-ERT2CreERT2) 的调节区下的 N 端和 C 端与突变型雌激素配体结合域 (ERT2) 融合。在VIPR2-ERT2CreERT2转基因小鼠中，Cre基因的mRNA在心脏中高表达。为了进一步揭示心脏特异性 Cre 表达，通过 X-gal 染色检查与 ROSA26-lacZ 报告小鼠交配的 VIPR2-ERT2CreERT2 小鼠。 X-gal染色结果显示，在用他莫昔芬治疗后，Cre依赖性重组仅发生在心脏中。综上所述，这些结果表明 VIPR2-ERT2CreERT2 转基因小鼠是揭示心脏中特定基因功能的有用模型。
Genetically engineered mouse models through gene deletion are useful tools for analyzing gene function. To delete a gene in a certain tissue temporally, tissue-specific and tamoxifen-inducible Cre transgenic mice are generally used. Here, we generated transgenic mouse with cardiac-specific expression of Cre recombinase fused to a mutant estrogen ligand-binding domain (ERT2) on both N-terminal and C-terminal under the regulatory region of human vasoactive intestinal peptide receptor 2 (VIPR2) intron and Hsp68 promoter (VIPR2-ERT2CreERT2). In VIPR2-ERT2CreERT2 transgenic mice, mRNA for Cre gene was highly expressed in the heart. To further reveal heart-specific Cre expression, VIPR2-ERT2CreERT2 mice mated with ROSA26-lacZ reporter mice were examined by X-gal staining. Results of X-gal staining revealed that Cre-dependent recombination occurred only in the heart after treatment with tamoxifen. Taken together, these results demonstrate that VIPR2-ERT2CreERT2 transgenic mouse is a useful model to unveil a specific gene function in the heart.