Calcium-modulated contractile proteins associated with the eucaryotic centrosome.
Calcium-modulated contractile proteins associated with the eucaryotic centrosome.
复制标题
与真核中心体相关的钙调节收缩蛋白。
DOI：
10.1002/cm.970060218
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发表时间：
1986
影响因子：
--
通讯作者：
Sanders,MA
中科院分区：
文献类型：
--
作者：
Salisbury,JL;Baron,AT;Coling,DE;Martindale,VE;Sanders,MA
识别 Tetraselmis striata 的 20,000 道尔顿分子量 (20 kd) 条纹鞭毛根蛋白的亲和纯化抗体已用于鉴定不同进化起源的其他真核生物中的抗原同源物。在绿藻 Tetraselmis 和 Chlamydomonas 及其无色亲戚 Polytomella 中，20-kd 同源物似乎与基体相关。这种情况最明显地以条纹和微管包覆类型的鞭毛根的形式出现。在培养的哺乳动物细胞（PtK2 和原代小鼠巨噬细胞系）中，鞭毛根蛋白同源物表现为基脚、中心粒周围原纤维和中心粒周围卫星。哺乳动物精子细胞还显示出与其基体相关的鞭毛根蛋白同源物。我们设想这些纤维状钙敏感收缩蛋白通过响应拓扑钙通量来改变中心粒或基体及其相关 MTOC 的方向。
Affinity‐purified antibodies that recognize the 20,000‐dalton molecular weight (20 kd) striated flagellar root protein ofTetraselmis striatahave been used to identify antigenic homologs in other eucaryotic organisms of diverse evolutionary origins. Among the green algae,TetraselmisandChlamydomonas, and their colorless relative,Polytomella, the 20‐kd homologs appear associated with basal bodies. This occurs most prominnently in the form of flagellar roots of both striated and microtubule subtended types. Among cultured mammalian cells (PtK2 and primary mouse macrophage cell lines), flagellar root protein homologs appear as basal feet, pericentriolar fibrils, and pericentriolar satellites. Mammalian sperm cells also show flagellar root protein homologs associated with their basal bodies. We envisage a functional role for these fibrous calcium‐sensitive contractile proteins in altering the orientation of centrioles or basal bodies with their associated MTOCs by responding to topological calcium fluxes.